摘要
目的探讨叉头框转录基因C1(FOXC1)和上皮钙黏蛋白(E-Cadherin)在肝癌组织中的表达,并分析与预后相关性及对癌细胞生物学行为的影响。方法将2017年4月—2022年4月在西北大学附属医院/西安市第三医院接受根治术治疗的85例原发性肝细胞癌患者为研究对象,均术中采集癌灶组织和癌旁组织,通过免疫组织化学法测定FOXC1、E-Cadherin的表达水平,并分析两者表达与患者预后的相关性。并选用细胞系HepG2,分为sh FOXC1、sh NC及空白对照组,通过平板克隆实验、Transwell实验测定细胞的增殖与侵袭能力。结果通过测定,本组病例肝癌组织中FOXC1蛋白和E-Cadherin阳性表达率分别为74.12%、28.24%,癌旁组织分别为43.53%、100.00%,差异存在统计学意义(P<0.05)。TNM分期是Ⅲ~Ⅳ期、低中分化、肿瘤直径≥3cm患者的FOXC1阳性表达率高,E-cadherin阳性表达率低,差异均存在统计学意义(P<0.05)。通过Spearman分析显示,在肝癌组织中FOXC1表达与预后呈负相关(P<0.05),E-Cadherin表达水平与预后呈正相关(P<0.05)。通过细胞实验,空白对照组、sh NC组FOXC1表达水平高于正常组,而E-cadherin表达低于正常组,差异均存在统计学意义(P<0.05)。在敲低FOXC1后,癌细胞增殖、侵袭等能力均显著减弱(P<0.05)。结论FOXC1、E-cadherin表达和肝癌治疗预后存在相关性,前者通过促进癌细胞增殖,并抑制后者表达促进上皮-间充质转化进程,增强肝癌细胞侵袭性,影响到临床预后。
Objective To investigate the expression levels of forkhead box transcription gene C1(FOXC1)and epithelial cadherin(E-Cadherin)in hepatocellular carcinoma(HCC)tissues,analyze the correlation of FOXC1 and E-Cadherin with prognosis,and explore the impact of FOXC1 and E-cadherin on the biological behavior of HCC cells.Methods A total of 85 patients with primary hepatocellular carcinoma who underwent radical resection in our hospital from April 2017 to April 2022 were selected for the study.Tumor focal tissues and adjacent tissues were collected during the operation.The expression levels of FOXC1 and E-Cadherin were determined by immunohistochemistry,and their correlation with prognosis of patients was analyzed.The HepG2 cell line was divided into sh FOXC1 group,sh NC group and blank control group.The proliferation and invasion ability of the cells were determined by plate cloning assay and Transwell assay.Results The positive expression rates of FOXC1 protein and E-Cadherin in HCC tissues were 74.12%and 28.24%,respectively.Meanwhile,in adjacent tissues,the rates were 43.53%and 100.00%,respectively.These differences were statistically significant(P<0.05).The positive expression rate of FOXC1 and E-cadherin was significantly higher in patients with TNM stageⅢ~Ⅳ,low to moderate differentiation,and tumor diameter≥3 cm.Conversely,the positive expression rates of E-cadherin was notably decreased in these patients.These differences were statistically significant(P<0.05).Spearman analysis revealed that FOXC1 expression was negatively correlated with prognosis(P<0.05),while E-Cadherin expression was positively correlated with prognosis(P<0.05).In the cell experiment,the expression of FOXC1 protein in the blank control group and sh NC group was higher than that in normal hepatocytes,and the expression of E-cadherin protein was lower than that in normal hepatocytes,with statistically significant differences(P<0.05).Downregulation of FOXC1 significantly impaired the proliferation and invasion of hepatocellular carcinoma cells(P<0.05).Conclusion The expression of FOXC1 and E-cadherin is correlated with the prognosis of hepatocellular carcinoma.FOXC1 promotes the proliferation of cancer cells and inhibits the expression of E-cadherin to promote the process of epithelial-mesenchymal transition,enhance the invasiveness of hepatocellular carcinoma cells,and affect clinical prognosis.
作者
聂媛媛
赵恒芳
王英娟
赵艳
NIE Yuan-yuan;ZHAO Heng-fang;WANG Ying-juan;ZHAO Yan(Department of Gastroenterology,Affiliated Hospital of Northwest University/,Xi'an Third Hospital,Shaanxi 710018,China;Department of Pediatrics,First Affiliated Hospital of Xi'an Medical College,Shaanxi 710077,China)
出处
《肝脏》
2023年第12期1446-1449,1454,共5页
Chinese Hepatology
关键词
叉头框蛋白C1
上皮钙黏素
肝癌
预后
生物学行为
Forkhead box protein C1
Epithelial cadherin
Cancer of the liver
The prognosis
Biological behavior
