摘要
为探究猪毛菜在表型、生理及分子层面对干旱胁迫的响应机制,采用控水法进行梯度干旱胁迫,对叶片进行解剖结构和生理指标测定及转录组测序。结果表明:干旱胁迫导致栅栏组织从致密变稀疏,晶簇先消失后增多;贮水组织厚度先增大后降低(P<0.05),主维管束面积和表皮厚度在28 d时显著增大,分别为(3099.72±151.88)μm^(2)和(23.73±0.68)μm(P<0.05),叶片厚度增大而叶面积减小(P<0.05);叶绿素含量逐渐降低(P<0.05),超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、脯氨酸和丙二醛(MDA)含量在28 d显著增大(P<0.05),H2O2含量在14 d显著增大(P<0.05),可溶性蛋白含量先增后降再增(P<0.05)。与0 d比较,干旱胁迫7、14和28 d的差异表达基因(DEGs)分别有103、1560和270个;基因本体(GO)富集分析表明各组合DEGs显著富集在蛋白质磷酸酶Ⅰ型复合体、膜的整体组件、氧化还原过程、氧化还原酶活性等条目中;京都基因与基因组百科全书(KEGG)富集分析表明各组合DEGs同时显著富集在氧化磷酸化代谢、核糖体和光传导等通路中。非生物胁迫通路DEGs分析表明,热激蛋白基因、GPX2、POD、SOD、CPK19、CIPK9、MAP激酶基因、蛋白质降解通路基因、UXS1和F3H等参与对干旱胁迫的响应调节。研究结果可为进一步研究猪毛菜抗旱机制提供参考。
This research investigated the phenotypic,physiological and molecular mechanism of Salsola collina response to drought stress.Plants were cultivated in pots under a controlled,declining soil moisture regime,falling from 80%to 50%of field water holding capacity over 28 days with sample collection at 0,7,14 and 28 days.It was found that the palisade tissue changed from dense to sparse and the crystal clusters disappeared initially and then increased across the 28 days of increasing drought stress.The thickness of aqueous tissue initially increased and then decreased(P<0.05).The area of the main vascular bundle and epidermis thickness after 28 days of drought stress[(3099.72±151.88)μm^(2) and(23.73±0.68)μm,respectively]were significantly increased compared to control[(2065.41±80.08)μm^(2) and(13.71±1.26)μm](P<0.05).Again with increasing drought stress in 28-day-old seedlings,the leaf thickness increased but the area decreased(P<0.05).Chlorophyll content decreased gradually(P<0.05),superoxide dismutase(SOD)and peroxidase(POD)activities,proline and malondialdehyde(MDA)contents increased significantly(P<0.05),H2O2 increased significantly at 14 days(P<0.05),soluble protein content increased initially,then decreased under medium stress and increased under high stress(P<0.05).Compared with day 0,there were 103,1560 and 270 differentially expressed genes(DEGs)detected at 7,14 and 28 days of drought stress,respectively.Gene ontology(GO)enrichment analysis showed that DEGs were significantly enriched in protein phosphatase typeⅠcomplex,integral components of membranes,oxidation-reduction process and oxidoreductase activity,among others.The Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that the genomes were significantly concentrated on oxidative phosphorylation,ribosome and phototransduction pathways.Abiotic stresses pathway DEGs analysis showed that heat shock protein genes,glutathione peroxidase 2(GPX2),POD,SOD,calcium-dependent protein kinase 19(CPK19)and CBL-interacting protein kinase 9(CIPK9),MAP kinase genes,transcription factor protein degradation pathway genes,UDP-glucuronate decarboxylase 1(UXS1)and flavanone 3-hydroxylase(F3H),among others,were involved in the regulation of drought stress responses.The results provide a reference point for further research on the drought resistance mechanism of S.collina.
作者
谭炯锐
查同刚
张泽宇
张晓霞
滕红梅
王玲丽
赵莉丽
王奥
王馨珧
TAN Jiong-rui;ZHA Tong-gang;ZHANG Ze-yu;ZHANG Xiao-xia;TENG Hong-mei;WANG Ling-li;ZHAO Li-li;WANG Ao;WANG Xin-yao(Department of Life Science,Yuncheng University,Yuncheng 044000,China;College of Soil and Water Conservation,Beijing Forestry University,Beijing 100083,China;The Third Construction Co.,Ltd.of China Construction First Construction Group,Beijing 100161,China;Beijing Badaling Forest Farm,Beijing 102112,China)
出处
《草业学报》
CSCD
北大核心
2024年第1期75-88,共14页
Acta Prataculturae Sinica
基金
中建股份科技研发计划(CSECE-2020-Z-5)
山西省基础研究计划项目(202103021223373,202203021211113)
特色农产品发展学科群项目(SKX-202219)资助。
关键词
干旱区植物
胁迫
解剖结构
生理特性
分子调控
arid zone plant
stress
anatomical structure
physiological property
molecular regulation
