摘要
为筛选出白城山新1号杨最佳增殖培养基配方,本试验采用正交试验法经组织培养对其进行繁育,以MS培养基为基础配方,将6-BA质量浓度、NAA质量浓度、大量元素含量、蔗糖质量浓度分别设置5个水平进行试验。经回归分析,确定MS+6-BA 0.30 mg·L^(-1)+NAA 0.05 mg·L^(-1)+蔗糖20.00 g·L^(-1)为白城山新1号杨组培增殖培养基优化配方。
In order to screen out the best multiplication culture medium of Populus davidiana×P.bolleana‘baichengshanxinyang No.1’,the orthogonal test method was used to breed it by tissue culture.Based on the MS culture medium,the mass concentration of 6-BA,the mass concentration of NAA,the content of macroelement and the mass concentration of sucrose were set at 5 levels respectively.Through regression analysis,MS+6-BA 0.30 mg·L^(-1)+NAA 0.05 mg·L^(-1)+sucrose 20.00 g·L^(-1)was determined as the optimal tissue culture.
作者
王利
张玉玲
包颖
岳龙
孙鹏
王新波
WANG Li;ZHANG Yuling;BAO Ying;YUE Long;SUN Peng;WANG Xinbo(Forestry Science Academy of Baicheng City,Baicheng 137000,China;Jilin Provincial Forestry Technology Popularizing Station,Changchun 130022,China;Forestry Bureau of Shulan City,Jilin 132000,China)
出处
《吉林林业科技》
2024年第1期13-16,共4页
Journal of Jilin Forestry Science and Technology
关键词
白城山新1号杨
组织培养
增殖培养
Populus davidiana×P.bolleana‘baichengshanxinyang No.1’
tissue culture
multiplication culture
