摘要
目的:探究Notch3/DLL4信号通路对颈部淋巴水囊瘤(CH)后淋巴管成熟发育的影响。方法:选取C57BL/6小鼠和C57BL/6背景Notch3基因敲入小鼠,将两种小鼠分为正常组(对照组)和Notch3基因敲入组(突变组)。选取F2代小鼠处死,取小鼠颈部淋巴结分别进行HE染色、Masson染色、RT-qPCR、Western blot、免疫组化检测。结果:HE及Masson染色结果显示,与对照组比,突变组小鼠的淋巴结构被显著破坏,细胞纤维化严重。RT-qPCR结果显示,与对照组比,突变组小鼠淋巴结中DLL4、Hes1和VEGFR3 mRNA表达显著下降(P<0.05)。Western blot结果显示,与对照组比,突变组小鼠淋巴结中DLL4、Hes1和VEGFR3蛋白表达显著下降(P<0.05)。免疫组化结果显示,与对照组相比,突变组小鼠淋巴结中DLL4、Hes1、Ang2、VEGFA和VEGFR3染色较浅,蛋白表达水平下降(P<0.05)。结论:Notch3/DLL4信号通路能够调控小鼠颈部CH的形成,其机制可能与淋巴管发育有关。
Objective:To investigate the effect of Notch3 signaling pathway on the maturation and development of fetal lymphatic vessels after cystic hygroma(CH).Methods:C57BL/6 mice and C57BL/6 background Notch3 gene knock-in mice were divided into a normal control group and a mutation group.Cervical lymph nodes were collected from F2 generation mice for HE staining,Masson staining,RT-qPCR,Western blot,and immunohistochemical analysis.Results:Compared with the control group.the results of HE and Masson staining showed that the cervical lymph nodes of the mutant mice were significantly disrupted and cellular fibrosis was severe;RT-qPCR results showed that the mRNA expression of DLL4,Hes1,and VEGFR3in lymphatic nodes of Notch3 mutant mice was significantly decreased,which had statistical difference(P<0.05);Western blot analysis showed that the expression of DLL4,Hes1,and VEGFR3 proteins in the lymphoid nodes of Notch3 mutant mice was remarkably decreased,indicating significant difference(P<0.05);immunohistochemical analysis showed that the expression of DLL4,Hes1,Ang2,VEGFA,and VEGFR3 in the lymphoid nodes of Notch3 mutant mice was decreased,with significant difference(P<0.05).Conclusion:Notch3 regulates cystic hygroma in mice,and its mechanism is likely to be through lymphatic vessel neogenesis.
作者
王荣跃
邱海凡
戴芬
楼文文
宫剑
谢爱兰
WANG Rongyue;QIU Haifan;DAI Fen;LOU Wenwen;GONG Jian;XIE Ailan(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China;Department of Laboratory Medicine,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
CAS
2024年第1期20-24,30,共6页
Journal of Wenzhou Medical University
基金
浙江省医药卫生科技计划项目(2020KY641)
温州市基础性科研项目(Y20210019)
浙江省自然科学基金项目(LY19HO40006)。
