摘要
目的:探讨LINC01006靶向miR-331-3p在多发性骨髓瘤(multiple myeloma,MM)进展中的功能。方法:RT-qPCR检测39例MM患者和22例正常对照者骨髓标本来源的浆细胞中LINC01006和miR-331-3p的表达。分别转染si-LINC01006、si-NC、miR-331-3p mimics、miR-NC、si-LINC01006+anti-miR-NC、si-LINC01006+anti-miR-331-3p至MM细胞U266,通过CCK-8、流式细胞术和Transwell法评估U266细胞抑制率、凋亡率和转移数。DIANA Tools网站预测LINC01006与miR-331-3p的相互作用,并通过双荧光素酶报告基因法进一步验证。结果:MM患者骨髓标本来源的浆细胞中LINC01006表达升高,miR-331-3p表达降低。转染si-LINC01006可增加细胞抑制率和凋亡率(P<0.05),减少细胞转移数(P<0.05),并上调miR-331-3p表达(P<0.05)。转染miR-331-3p mimics可增加细胞抑制率和凋亡率(P<0.05),减少细胞转移数(P<0.05)。miR-331-3p与LINC01006直接结合。转染anti-miR-331-3p显著逆转转染si-LINC01006对MM细胞抑制率、凋亡率和转移数的影响。结论:沉默LINC01006通过促进miR-331-3p表达来抑制MM细胞增殖和转移,诱导凋亡,从而抑制MM进展。
Objective:To investigate the role of LINC01006 targeting miR-331-3p in the development of multiple myeloma(MM).Methods:LINC01006 and miR-331-3p expression in plasma cells from bone marrow specimens of 39 MM patients and 22 normal controls was calculated by RT-qPCR.si-LINC01006,si-NC,miR-331-3p mimics,miR-NC,si-LINC01006+anti-miR-NC and si-LINC01006+anti-miR-331-3p were transfected into MM cell U266,respectively.CCK-8,Flow cytometry and Transwell method were applied to evaluate U266 cell inhibition rate,apoptosis rate and metastasis number.The DIANA Tools website was used to predict the interaction between LINC01006 and miR-331-3p,and which was further verified by the dual luciferase reporter gene method.Results:The expression of LINC01006 was increased,while the expression of miR-331-3p was decreased in plasma cells from bone marrow specimens of MM patients.Transfection of si-LINC01006 could increase the cell inhibition rate and apoptosis rate(P<0.05),reduce the number of cell metastases(P<0.05),and up-regulate miR-331-3p expression(P<0.05).Transfection of miR-331-3p mimics could increase the cell inhibition rate and apoptosis rate(P<0.05),and reduce the number of cell metastases(P<0.05).miR-331-3p was found to directly bind with LINC01006.Transfection of anti-miR-331-3p significantly reversed the effect of transfection of si-LINC01006 on the inhibition rate,apoptosis rate and metastasis number of MM cells.Conclusion:Silencing LINC01006 inhibits the proliferation and metastasis and induces apoptosis of MM cells by promoting miR-331-3p expression,thereby inhibiting the development of MM.
作者
李行
李强
朱品伟
柘娜娜
葛金丽
周震沧
刘兆玉
陈登科
LI Hang;LI Qiang;ZHU Pinwei;ZHE Nana;GE Jinli;ZHOU Zhencang;LIU Zhaoyu;CHEN Dengke(Department of Hematology,The First People's Hospital of Zunyi City(The Third Affiliated Hospital of Zunyi Medical University),Guizhou Zunyi 563000,China)
出处
《现代肿瘤医学》
CAS
2024年第1期7-12,共6页
Journal of Modern Oncology
基金
贵州省遵义市科技计划项目[编号:遵市科合HZ(2022)96号]。
