摘要
为进一步减少氮污染物排放,满足城市污水处理厂深度脱氮提标改造的需求,本研究使用新型活性砂滤罐以二沉池出水为对象进行深度脱氮试验,考察不同调控因子对总氮(TN)去除效果以及微生物群落结构的影响。结果表明,最优水力停留时间(HRT)为0.28 h,最佳碳氮比(C/N)为6:1,在此条件下出水TN浓度低于1.5 mg/L,优于现有深度脱氮工艺;为节约运行成本,HRT可调整至0.13~0.23 h,C/N参数不变,TN出水浓度低于4 mg/L。使用乙酸钠时微生物的响应时间比使用甲醇时更短,但高C/N条件下使用甲醇时脱氮效果比使用乙酸钠更好。16s rRNA高通量测序结果表明,系统主要脱氮功能微生物为Saccharibacteria_genera_incertae_sedis、Anaerolineaceae、Longilinea、Rhodocyclaceae、Chlorobium、Thiobacillus、Betaproteobacteria等,Anaerolineaceae主要聚集于装置底部。反冲洗会导致中下部生物膜量较高,但滤床循环运行的方式能将装置底部的反硝化菌转移至滤料顶部,使微生物在装置内的分布更加均匀,从而保证较好的深度脱氮效果。
To further reduce the discharge of nitrogen pollutants and meet the needs of deep denitrification and upgrading of urban sewage treatment plants,this study conducted a deep denitrification experiment using a new type of activated sand filter tank as the object of secondary sedimentation tank effluent,to investigate the effects of different regulatory factors on total nitrogen(TN)removal efficiency and microbial community structure.The results showed that the optimal hydraulic retention time(HRT)is 0.28 h,and the optimal carbon-nitrogen ratio(C/N)is 6:1.Under these conditions,the TN concentration in the effluent is less than 1.5 mg/L,which is superior to the existing deep denitrification process.To save operating costs,HRT can be adjusted to 0.13~0.23 h,C/N parameters remain unchanged,and TN effluent concentration is below 4 mg/L.The response time of microorganisms is shorter when using sodium acetate than when using methanol,but the denitrification effect is better when using methanol under high C/N conditions than when using sodium acetate.The results of 16s rRNA high-throughput sequencing showed the main denitrogenation microorganisms in the system are Saccharibacteria_genera_incertae_Sedis,Anaerolineaceae,Longilinea,Rhodococclaceae,Chlorobium,Thiobacillus,Betaproteobacteria,etc.Anaerolineaceae mainly concentrates at the bottom of the device.Backwashing will lead to a high amount of biofilm in the middle and lower parts,but the circulating operation of the filter bed can transfer the denitrification bacteria at the bottom of the device to the top of the filter material,so that the distribution of microorganisms in the device is more uniform,to ensure a better effect of deep denitrification.
作者
李桂荣
李智
乔海兵
贾胜勇
方芳
LI Guirong;LI Zhi;QIAO Haibing;JIA Shengyong;FANG Fang(College of Ecology and Environment,Zhengzhou University,Zhengzhou 450001,China;Henan Urban and Rural Planning and Design Research Institute Co.,Ltd,Zhengzhou 450044,China;Hainan Lvjing Hi Tech Environmental Protection Co.,Ltd,Haikou 570216,China)
出处
《水处理技术》
CAS
CSCD
北大核心
2024年第1期47-54,共8页
Technology of Water Treatment
基金
河南省重点研发与推广专项(科技攻关)(212102310071,212102110112)资助。
关键词
活性砂滤罐
深度脱氮
微生物
高通量测序
activated sand filter tank
deep denitrification
microorganisms
high throughput sequencing
