摘要
本试验以多肉植物枝干番杏、生石花、鲸波为材料,采用离体培养法研究蔗糖、硼酸、氯化钙浓度及培养温度对花粉萌发率的影响,优化花粉离体萌发条件,并结合TTC染色法对3种多肉植物的花粉活力进行测定。结果表明:在4个因素中,温度对多肉植物花粉萌发率影响较大,氯化钙影响较小,蔗糖和硼酸对多肉植物花粉萌发率的影响大小因植物种类而异;从花粉萌发率的统计结果来看,培养基中添加12%蔗糖、0.01%~0.02%硼酸、0.01%~0.03%氯化钙,放置在20℃下培养6 h,较适合番杏科多肉植物花粉的萌发。离体培养法和TTC染色法两种检测方法均显示,生石花的花粉活力最高,枝干番杏次之,鲸波最低。
In this experiment,three succulent plants such as Dicrocaulon ramulosum(L.Bolus)Ihlenf.,Lithops karasmontana and Faucaria bosscheana var.haagei were used as experimental materials,the effects of sucrose,boric acid and calcium chloride concentrations,and culture temperature on pollen germination rate were studied by using in vitro culture method,and the conditions for in vitro pollen germination were optimized.In addition,the pollen vitality of three succulent plants was measured by TTC staining method.The results showed that temperature had a significant impact on the pollen germination rate of succulent plants among the four factors,while calcium chloride had less effect,the effects of sucrose and boric acid on the pollen germination rate of succulent plants varied depending on the plant species.From the statistical results of pollen germination rate,it could be seen that adding 12%sucrose,0.01%-0.02%boric acid,0.01%-0.03%calcium chloride to the culture medium and culturing it at 20℃for 6 hours was more suitable for the germination of pollen from succulent plants in the Aizoaceae family.Both in vitro culture method and TTC staining method showed that the pollen vitality of Lithops karasmontana was the highest,followed by Dicrocaulon ramulosum(L.Bolus)Ihlenf.,and that of Faucaria bosscheana var.haagei was the lowest.
作者
倪文婷
席豪镁
陈雨萱
孟彤
黄梁施娜
李素华
NI Wenting;XI Haomei;CHEN Yuxuan;MENG Tong;HUANG Liangshina;LI Suhua(College of Information Engineering,Suqian University,Suqian Jiangsu 223800)
出处
《现代农业科技》
2024年第1期97-100,共4页
Modern Agricultural Science and Technology
基金
宿迁学院大学生实践创新训练项目(202214160066H)
宿迁市科技计划项目(自然科学)(K202018)
宿迁市科技计划项目(M202001)
宿迁学院学科建设项目(2021ZDJS04)。
关键词
多肉植物
番杏科
花粉活力
离体萌发
TTC染色
succulent plant
Aizoaceae family
pollen vitality
in vitro germination
TTC staining
