摘要
目的探讨脂肪因子C1q/肿瘤坏死因子相关蛋白6(CTRP6)和CTRP9在妊娠期糖尿病(GDM)患者血清中的表达及应用价值。方法选取2019年10月—2020年10月在南京医科大学附属淮安第一医院门诊确诊的35名GDM患者(GDM组),另选取同期产检正常的37名孕妇(对照组)。比较2组患者的一般临床资料、血脂、肝肾功能、空腹血糖(FPG)、空腹胰岛素(FINS)、稳态模型胰岛素抵抗指数(HOMA-IR)等。采用酶联免疫吸附法(ELISA)检测2组血清CTRP6、CTRP9水平,运用液态悬浮芯片(Luminex-xMAP)技术检测IL-6和肿瘤坏死因子α(TNF-α)水平。采用受试者工作特征(ROC)曲线分析CTRP6和CTRP9的诊断价值。结果GDM组血清CTRP6、CTRP9、IL-6和TNF-α水平较对照组显著升高(P<0.001)。Pearson相关性分析显示:血清CTRP6与FPG(r=0.280,P=0.017)和HOMA-IR(r=0.250,P=0.035)呈正相关,血清CTRP9与FPG(r=0.559,P<0.001)、FINS(r=0.253,P=0.032)、HOMA-IR(r=0.382,P=0.001)、IL-6(r=0.283,P=0.016)和TNF-α(r=0.266,P=0.024)呈正相关。ROC曲线分析显示:血清CTRP6和CTRP9单独和联合检测诊断GDM的曲线下面积(AUC)分别为0.914、0.914和0.974。结论GDM患者血清中CTRP6和CTRP9表达水平明显升高,且与胰岛素抵抗存在相关性。CTRP6和CTRP9对GDM患者的诊断具有一定价值,联合诊断价值更高。
Objective To investigate the expression and clinical significance of serum adipocytokines C1q/tumor necrosis factor-related protein(CTRP)6 and CTRP9 in patients with gestational diabetes mellitus(GDM).Methods A total of 35 pregnant women who were admitted to the Affiliated Huai′an First People′s Hospital of Nanjing Medical Uni-versity from October 2019 to October 2020 and diagnosed with GDM were selected and set a GDM group.Meanwhile,an-other 37 normal pregnant women who underwent examination at the same period were included in a control group.Both groups were compared for general information,blood lipid,hepatic and renal function,fasting plasma glucose(FPG),fasting insulin(FINS)and homeostasis model insulin resistance index(HOMA-IR).The levels of serum CTRP6 and CTRP9 were measured by enzyme-linked immunosorbent assay(ELISA).The amounts of IL-6 and TNF-αwere exam-ined by Luminex-xMAP technology.The diagnostic value of CTRP6 and CTRP9 was analyzed by the receiver operating characteristic(ROC)curve.Results The levels of serum CTRP6,CTRP9,IL-6 and TNF-αwere obviously higher in the GDM group,compared with the control group(P<0.001).Pearson correlation analysis indicated that serum CTRP6 was positively correlated with FPG(r=0.280,P=0.017)and HOMA-IR(r=0.250,P=0.035).Furthermore,CTRP9 was positively correlated with FPG(r=0.559,P<0.001),FINS(r=0.253,P=0.032),HOMA-IR(r=0.382,P=0.001),IL-6(r=0.283,P=0.016),and TNF-α(r=0.266,P=0.024).According to ROC curve anal-ysis,the area under the curve(AUC)of serum CTRP6 and CTRP9 alone and their combination in predicting GDM was 0.914,0.914 and 0.974,respectively.Conclusions The levels of serum CTRP6 and CTRP9 in GDM patients are ele-vated,which may be related to insulin resistance.CTRP6 and CTRP9 have a certain value for the diagnosis of GDM pa-tients,while the combined detection of CTRP6 and CTRP9 can improve the predictive value of GDM to a certain extent.
作者
刘瑜婷
胡长军
尹路路
曹慧敏
石敏
LIU Yuting;HU Changjun;YIN Lulu;CAO Huimin;SHI Min(Department of Endocrinology,the Affiliated Huai′an First People′s Hospital of Nanjing Medical University,Huai′an,Jiangsu 223300,China)
出处
《徐州医科大学学报》
CAS
2023年第12期882-886,共5页
Journal of Xuzhou Medical University
基金
国家自然科学基金(81700723)。
关键词
妊娠期糖尿病
C1q/肿瘤坏死因子相关蛋白
炎症因子
胰岛素抵抗
糖调节受损
gestational diabetes mellitus
C1q/tumor necrosis factor-related protein
inflammatory factors
insulin resistance
impaired glucose regulation
