摘要
目的分析山东省济南市和济宁市白纹伊蚊(Aedes albopictus)线粒体细胞色素c氧化酶的一个亚基I基因(mitochondrial cytochrome c oxidase subunitⅠ,mtDNA-COⅠ),揭示白纹伊蚊不同亚群体间的遗传差异、亲缘关系,探讨白纹伊蚊遗传多样性与地理分布之间的关系。方法分别从济南市历城区和济宁市任城区采集白纹伊蚊样本,利用PCR技术扩增白纹伊蚊种群的mtDNA-COⅠ基因序列,结果通过NCBI的BLAST比对工具进行比对,运用BioEdit 7.0、DnaSP v6和PopART 1.7软件进行数据分析,计算白纹伊蚊遗传多样性参数并绘制单倍型网络图。结果对山东省济南市和济宁市的白纹伊蚊mtDNA-COⅠ基因进行了基因测序,分别获得了86条和84条基因片段。与BLAST比对结果显示,全部序列存在5个突变位点,核苷酸多样性(nucleotide diversity,Pi)为0.00097,G+C含量为33.4%,表现出线粒体DNA特性。与济宁市相比,济南市的白纹伊蚊种群在核苷酸多样性和单倍型多样性上均较高,核苷酸差异也更大,但这些差异无统计学意义。济南市和济宁市的白纹伊蚊种群各自拥有6种单倍型,其中3种是共同存在的,而另外3种仅在济南市的种群中发现。种群结构分析结果显示,济南与济宁两地的白纹伊蚊种群的群体间核苷酸差异很小。结论mtDNA-COⅠ基因可用于物种鉴定以及研究白纹伊蚊种群遗传多样性的分子标志,为更有效的分类和防控策略提供支持。
Objective This study aimed to analyze the mitochondrial cytochrome c oxidase subunit I(mtDNA-COⅠ)gene in Aedes albopictus populations from Ji'nan and Ji'ning Cities in Shandong Province,in order to reveal the genetic differences and phylogenetic relationships among different subpopulations and to investigate the relationship between genetic diversity and geographical distribution of Aedes albopictus.Method In this study,Ae.Albopictus mosquitoes were collected from Licheng District in Ji'nan City and Rencheng District in Ji'ning City.The sequences of mtDNA-COⅠgene of Ae.albopictus populations was amplified by PCR,and the results were aligned and compared using the BLAST tool from NCBI.Data analysis was then conducted using the software of BioEdit 7.0,DnaSP v6 and PopART 1.7.Genetic diversity parameters for Aedes albopictus,and a haplotype network was constructed.Results Gene sequencing of the mtDNA-COⅠgene in Aedes albopictus populations from Ji'nan and Ji'ning cities in Shandong Province resulted in 86 and 84 gene fragments,respectively.BLAST comparison revealed five mutation sites in all the sequences,with a nucleotide diversity(Pi)of 0.00097 and a G+C content of 33.4%,reflecting the characteristics of mitochondrial DNA.Compared to Ji'ning,the Ae.albopictus population in Ji'nan exhibited higher nucleotide diversity and haplotype diversity,as well as greater nucleotide differences,though these differences were not statistically significant.Furthermore,both cities had six haplotypes of Aedes albopictus,with three shared haplotypes and three unique haplotypes found only in the population of Ji'nan City.Population structure analysis revealed minimal nucleotide differences between Ae.albopictus populations of Ji'nan and Ji'ning.Conclusions The mtDNA-COⅠgene can be used as a molecular marker for species identification and for studying genetic diversity of Ae.albopictus populations,thereby providing support for more effective classification and control strategies.
作者
刘鲁宏
李黎明
刘烁
LIU Luhong;LI Liming;LIU Shuo(Ji'ning Center for Disease Control and Prevention,Ji'ning,Shangdong 272033,China)
出处
《中国热带医学》
2023年第12期1289-1293,共5页
China Tropical Medicine
