摘要
大豆是重要的油料作物,同时也是人类食用植物蛋白及畜牧业饲料蛋白的主要来源,在国家粮食结构和粮食安全中占有重要地位。利用简单高效的遗传定位方法,对大豆主要农艺性状进行相关基因挖掘,开发紧密连锁分子标记,有利于加快大豆的分子标记辅助选择及分子设计育种进程。集群分离分析法(BSA,bulked segregant analysis)是一种利用样本混池的建库方式对极端性状进行QTL定位的方法,因其具有“快速、准确、经济、实用”的特点,已成为当下应用较为广泛的基因定位方法。随着高通量测序技术的兴起,基于全基因组重测序的BSA方法更为广泛地应用在粮油作物、蔬菜花卉等物种中,并且成功定位出许多农艺性状相关的基因。本文简要介绍了BSA方法及流程步骤,总结了BSA在大豆农艺性状、抗逆性状以及雄性不育性状遗传定位中研究进展,并讨论了下一代测序(NGS,next-generation sequencing)背景下BSA的机遇与挑战,以及BSA在大豆分子标记辅助选择(MAS)育种中发展趋势,以期为高产优质大豆品种的选育提供重要的理论基础。
Soybean is not only an important oil crop,but also the main source of plant protein for human consumption and feed protein for animal husbandry,which plays an important role in the national food structure and food security.By using a simple and efficient genetic mapping method,the related genes of the main agronomic characters of soybean are mined and closely linked molecular markers are developed,which is helpful to speed up the process of molecular marker-assisted selection and molecular design breeding of soybean.Bulked Segregant Analysis(BSA)is a method to identify the gene loci through using the DNA pools with extreme traits.Due to its characteristics of fast,accurate,economical and practical,BSA has become a widely-applied method in genetic mapping.Taking advantage of high-throughput sequencing technology,BSA based on whole-genome resequencing has been widely applied in isolation of many genes underlying agronomic traits in grain and oil crops,vegetables,flowers and other species.This study briefly introduced the methods and procedures of BSA,summarized the research progress in genetic mapping of agronomic traits,stress resistance traits and male sterility traits in soybean,and further discussed the opportunities and challenges of BSA under the background of NGS,as well as the development trend of BSA in soybean molecular marker assisted selection(MAS)breeding.This study will provide theoretical basis for the cultivation of high quality soybean varieties.
作者
师立松
张艺龄
刘方
颜硕
张孟茜
赵璇
李红雨
牛宁
李占军
SHI Lisong;ZHANG Yiing;LIU Fang;YAN Shuo;ZHANG Mengxi;ZHAO Xuan;LI Hongyu;NIU Ning;LI Zhanjun(Shijiazhuang Academy of Agricultural and Forestry Sciences/Hebei Academy of Provincial Soybean Industry Technology,Shijiazhuang 050041)
出处
《植物遗传资源学报》
CSCD
北大核心
2024年第1期1-12,共12页
Journal of Plant Genetic Resources
基金
石家庄市科学技术研究与发展计划项目(221490112A)
河北省大豆产业技术研究院建设补助经费项目(225790287H)
河北省科技计划项目现代种业科技创新专项(21326333D)。
