摘要
目的探讨慢性阻塞性肺疾病(COPD)急性加重期患者外周血单个核细胞中细胞因子信号抑制蛋白-1(SOCS-1)、Toll样受体4(TLR4)mRNA水平及血清心肌肌钙蛋白T(cTnT)、尿酸水平变化。方法收集COPD急性加重期(组)患者70例,COPD稳定期(组)患者40例,对照组健康志愿者40名。检测外周血单个核细胞SOCS-1、TLR4 mRNA水平及血清cTnT、尿酸浓度;行肺功能检查并记录相关指标(FEV1、FEV1%、FEV1/FVC%)。对COPD急性加重期患者进行1 a随访,分为预后不良组和预后良好组。对外周血单个核细胞SOCS-1、TLR4 mRNA水平、血清cTnT、尿酸浓度行Pearson相关性分析,并对COPD急性加重期患者预后评估价值进行ROC曲线分析。结果COPD急性加重期组SOCS-1 mRNA表达水平明显低于COPD稳定期组、对照组,TLR4 mRNA水平及血清cTnT、尿酸浓度明显高于COPD稳定期组和对照组(均P<0.01)。COPD急性加重期组FEV1、FEV1%、FEV1/FVC%明显低于COPD稳定期组和对照组(P<0.05)。COPD急性加重期患者FEV1/FVC%与外周血单个核细胞SOCS-1 mRNA表达水平呈正相关关系(P<0.01),与外周血单个核细胞TLR4 mRNA水平及血清cTnT、尿酸浓度呈负相关关系(P<0.01)。预后不良组SOCS-1 mRNA水平明显低于预后良好组,TLR4 mRNA水平及血清cTnT、尿酸浓度明显高于预后良好组(P<0.05)。外周血单个核细胞SOCS-1、TLR4 mRNA水平及血清cTnT、尿酸联合检测对COPD急性加重期患者预后具有较高的评估价值。结论COPD急性加重期患者SOCS-1低表达,TLR4、cTnT、尿酸高表达,且与肺功能水平密切相关,联合检测对患者预后具有较高的评估价值。
Objective To investigate the change of cytokine-induced suppressor of cytokine signaling protein 1(SOCS-1),Toll-like receptor 4(TLR4)mRNA,serum cardiac troponin T(cTnT),and uric acid levels in peripheral blood mononuclear cells of patients with acute exacerbation of chronic obstructive pulmonary disease(COPD).Methods Collect 70 patients in the acute exacerbation phase of COPD,40 patients in the stable phase of COPD,and 40 healthy volunteers in the control group.Detect the levels of SOCS-1 and TLR4 mRNA in peripheral blood mononuclear cells,as well as serum cTnT and uric acid concentrations;Perform lung function tests and record relevant indicators(FEV1,FEV1%,FEV1/FVC%).Patients with acute exacerbation of COPD were followed up for 1 year and divided into poor prognosis group and good prognosis group.Pearson correlation analysis was conducted peripheral blood mononuclear cell SOCS-1,TLR4 mRNA,as well as serum cTnT and uric acid concentrations.ROC curve analysis was conducted to evaluate the prognostic value of acute exacerbation of COPD.Results The expression level of SOCS-1 mRNA in the acute exacerbation group significantly lower than that in the stable COPD group and the control group,while TLR4 mRNA,serum cTnT and uric acid conce-ntrations were significantly higher in the acute exacerbation group compared to the stable COPD group and the control group(P<0.01).FEV1,FEV1%and FEV1/FVC%in the acute exacerbation group were significantly lower than those in the stable COPD group and the control group(P<0.05).In patients with acute exacerbation of COPD,FEV1/FVC%was positively correlated with the expression level of SOCS-1 mRNA in PBMCs(P<0.01),and negatively correlated with the expression level of TLR4 mRNA in PBMCs,as well as serum cTnT and uric acid concentrations(P<0.01).The expression level of SOCS-1 mRNA in the poor prognosis group was significantly lower than that in the good prognosis group,while TLR4 mRNA,serum cTnT and uric acid conce-ntrations were significantly higher in the poor prognosis group compared to the good prognosis group(P<0.05).Combined detection of SOCS-1 and TLR4 mRNA in PBMCs,as well as serum cTnT and uric acid,has a high value in predicting prognosis of patients with acute exacerbation of COPD.Conclusion Patients with acute exacerbation of COPD have low expression of SOCS-1,high expression levels of TLR4,cTnT and uric acid,which are closely related to lung function levels.Combined detection has a high value in assessing prognosis of patients.
作者
云俊杰
徐影
YUN Junjie;XU Ying(Affiliated Ninth People’s Hospital,School of Medicine of Shanghai Jiaotong University,Shanghai 200021,China)
出处
《北华大学学报（自然科学版）》
CAS
2024年第2期185-190,共6页
Journal of Beihua University（Natural Science）
基金
上海市卫健委卫生行业临床研究专项课题(202140222)。
