摘要
西瓜未受精子房离体培养是诱导单倍体的有效方法,可缩短育种时间。为建立高效稳定的单倍体诱导体系,提高单倍体诱导率,以大果型品种雪龙1号、雪龙3号,中果型品种早蜜、红都2号和小果型品种小玉9号和黄小玉的6个杂交一代(2n=2x=22)西瓜的未受精子房为试验材料,研究不同植物生长调节剂种类和质量浓度对西瓜单倍体诱导率的影响。结果表明,6个不同基因型品种在2 mg·L^(-1)6-BA+1 mg·L^(-1)NAA和2 mg·L^(-1)6-BA+2 mg·L^(-1)NAA的诱导培养基中均能诱导出胚状体,除黄小玉以外,均获得了单倍体再生植株;其中,红都2号的胚状体诱导率最高,达63.89%;其次是早蜜,诱导率为55.56%。
Unfertilized ovary culture of watermelon is an effective method to induce haploids,which can shorten the breeding time.In order to establish an efficient and stable haploid induction system and improve the haploid induction rate,the unfertilized ovaries of six watermelon hybrids(2n=2x=22)of large-fruited variety Xuelong 1 and Xuelong 3,medium-fruited variety Zaomi,Hongdu 2,and small-fruited variety Xiaoyu 9,Huangxiaoyu were used as experimental materials.The effects of different plant growth regulators on the haploid induction rate of watermelon were studied.The results showed that six different genotypes of watermelon were induced in the induction medium 2 mg·L^(-1)6-BA+1 mg·L^(-1)NAA,2 mg·L^(-1)6-BA+2 mg·L^(-1)NAA,and haploid regeneration plants were obtained except for Huangxiaoyu.Among them,Hongdu 2 had the highest induction rate of 63.89%,followed by Zaomi with an induction rate of 55.56%.
作者
戴玉娟
龚诗琦
孙翔宇
陈浩杰
熊程
孙小武
戴思慧
DAI Yujuan;GONG Shiqi;SUN Xiangyu;CHEN Haojie;XIONG Cheng;SUN Xiaowu;DAI Sihui(Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education/College of Horticulture,Hunan Agricultural University,Changsha 410128,Hunan,China)
出处
《中国瓜菜》
CAS
北大核心
2024年第1期18-24,共7页
China Cucurbits And Vegetables
基金
国家重点研发计划项目(2020YFD1000300)
国家西甜瓜产业技术体系(CARS-25)
湖南省教育厅科研项目(19B271)。
关键词
西瓜
植物生长调节剂
单倍体诱导
Watermelon
Plant growth regulator
Haploid induction
