摘要
核孔复合体是物质穿梭核膜的通道。核孔蛋白能影响核质和胞质间的物质交换,而核质运输具有调控生殖细胞进入雌性或雄性分化途径的能力。性腺体细胞衍生因子(gonadal soma-derived factor,gsdf)能调控青鳉Oryzias latipes雄性生殖细胞,并在有丝分裂最后一轮之前开启或关闭配子发生中起关键作用。通过RT-qPCR实验发现nup 58(nucleoporin 58)在野生型青鳉精巢中的表达高于卵巢。对青鳉性腺转录组数据分析发现,nup 58在gsdf敲除后精巢中的表达会显著升高。免疫荧光实验结果显示,nup 58在配子发生不同阶段的所有生殖细胞中都有表达。我们的研究显示Nup58作为一种核孔蛋白,受gsdf信号的调控,并与雄性生殖细胞的增殖密切相关。由Nup58核孔蛋白协调的核质运输在生殖细胞性别分化中的作用和动态变化值得进一步探讨。
Nuclear pore complexes(NPCs)form channels to transport materials across the nuclear membrane.Nucleoporin can affect the material exchange between nucleoplasm and cytoplasm,and the nucleoplasm transport has the ability to coordinates germ cells into female or male differentiation pathways.Gonadal soma-derived factor(Gsdf)can regulate the male germ cells of Oryzias latipes and play a key role in switching on/off gametogenesis before the last turn of mitosis.The expression of nup 58(nucleoporin 58)was higher in normal testis than that in normal ovary detected by RT-qPCR.Moreover,compared with normal testicular transcriptome data of medaka(Oryzias latipes),the expression of nup 58 in gsdf deficient testis was found to significantly increase by the RNA-seq.The results of immunofluorescence assay showed that nup 58 was expressed in all germ cells at different stages of gametogenesis in both wild type and gsdf-knockout medaka gonads.This research shows that Nup58,as a nucleoporin,is regulated by gsdf signal and is closely related to the proliferation of male germ cells.Nup58 participates in dynamic events of nuclear cytoplasmic transport during sexual differentiation,and its synergistic effects need to be explored.
作者
李雅园
王光幸
李新闻
郭振华
关桂君
LI Yayuan;WANG Guangxing;LI Xinwen;GUO Zhenhua;GUAN Guijun(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)
出处
《广西师范大学学报（自然科学版）》
CAS
北大核心
2024年第1期168-179,共12页
Journal of Guangxi Normal University:Natural Science Edition
基金
国家重点研发计划(2018YFD0900601)。
关键词
青鳉
性腺
性别分化
转录组
medaka
gonad
sex differentiation
transcriptome
