摘要
Conventional blood sampling for glucose detection is prone to cause pain and fails to continuously record glucose fluctuations in vivo.Continuous glucose monitoring based on implantable electrodes could induce pain and potential tissue inflammation,and the presence of reactive oxygen species(ROS)due to inflammationmay affect glucose detection.Microneedle technology is less invasive,yet microneedle adhesion with skin tissue is limited.In this work,we developed a microarrow sensor array(MASA),which provided enhanced skin surface adhesion and enabled simultaneous detection of glucose and H_(2)O_(2)(representative of ROS)in interstitial fluid in vivo.The microarrows fabricated via laser micromachining were modified with functional coating and integrated into a patch of a three-dimensional(3D)microneedle array.Due to the arrow tip mechanically interlocking with the tissue,the microarrow array could better adhere to the skin surface after penetration into skin.The MASA was demonstrated to provide continuous in vivo monitoring of glucose and H_(2)O_(2) concentrations,with the detection of H_(2)O_(2) providing a valuable reference for assessing the inflammation state.Finally,the MASA was integrated into a monitoring system using custom circuitry.This work provides a promising tool for the stable and reliable monitoring of blood glucose in diabetic patients.
基金
This work was financially supported by the National Key R&D Program of China(Nos.2021YFF1200700 and 2021YFA0911100)
the National Natural Science Foundation of China(Nos.32171399,32171456,and T2225010)
the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515012261)
the Science and Technology Program of Guangzhou,China(No.202103000076)
the Fundamental Research Funds for the Central Universities,Sun Yat-Sen University(No.22dfx02),and Pazhou Lab,Guangzhou(No.PZL2021KF0003)
FML would like to thank the National Natural Science Foundation of China(Nos.32171335 and 31900954)
JL would like to thank the National Natural Science Foundation of China(No.62105380)
the China Postdoctoral Science Foundation(No.2021M693686)
QQOY would like to thank the China Postdoctoral Science Foundation(No.2022M713645).
