摘要
目的分析健康献血者血浆中可溶性糖蛋白A(glycoprotein A,GPA)表达量与抗-M及抗-“Mia”抗体的相关性。方法收集2022年2月9日~2023年2月15日的健康献血者血浆:不规则抗体阴性的NN型(Ⅰ组,n=118)与MM型(Ⅱ组,n=51),抗-M抗体阳性的NN型(Ⅲ组,n=145)与抗-“Mia”抗体阳性的随机型(Ⅳ组,n=87),分别检测4组人不同个体血浆中GPA的含量,t检验分析GPA表达量的差异。结果Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组血浆中GPA平均含量分别为:9.941±0.252,10.97±0.256,5.139±0.129和4.28±0.139ng/ml。Ⅰ组与Ⅱ组的GPA平均含量较高,Ⅲ组与Ⅳ组的GPA平均含量较低,显示GPA平均含量在不规则抗体阴性(Ⅰ组与Ⅱ组)的血浆中较高,在抗-M与抗-“Mia”抗体阳性(Ⅲ与Ⅳ组)的血浆中较低,差异具有统计学意义(均P<0.01)。结论含有抗-M与抗-“Mia”抗体的健康献血者血浆中GPA含量明显低于抗体阴性的群体。该研究结果为深入探讨血浆中GPA是否有中和抗-M与抗-“Mia”抗体的能力,提高疾病诊断与安全输血打下基础。
Objective To analyze the correlation between the expression of soluble glycoprotein A(GPA)in plasma of healthy blood donors and anti-M and anti-“Mia”antibodies.Methods Plasma from healthy donors from February 9,2022 to February 15,2023 was collected:irregular antibody-negative NN type(groupⅠ,n=118)and MM type(groupⅡ,n=51),anti-M antibody positive NN type(groupⅢ,n=145)and anti-“Mia”antibody positive companion type(groupⅣ,n=87),the GPA content in plasma of different individuals in 4 groups was detected,and the difference in GPA expression was analyzed by t-test.Results The average plasma GPA contents in groupsⅠ,Ⅱ,ⅢandⅣwere 9.941±0.252,10.97±0.256,5.139±0.129 and 4.28±0.139ng/ml,respectively.The average GPA content of groupsⅠandⅡwas higher,and the average GPA content of groupsⅢandⅣwas lower,and the differences were statistically significant(all P<0.01).Conclusion The GPA content in plasma of healthy donors with anti-M and anti-“Mia”antibodies was significantly lower than that of the antibody-negative group.The results of this study lay a foundation for further investigation of whether GPA in plasma has the ability to neutralize anti-M and anti-“Mia”antibodies,improve disease diagnosis and safe blood transfusion.
作者
梁延连
邬林枫
唐雄驰
苏宇清
吴凡
梁爽
孙丽艳
LIANG Yanlian;WU Linfeng;TANG Xiongchi;SU Yuqing;WU Fan;LIANG Shuang;SUN Liyan(Institute of Transfusion Medicine,Shenzhen Blood Center,Guangdong Shenzhen 518040,China;Pinnan County People’s Hospital,Guangxi Pingnan 537300,China)
出处
《现代检验医学杂志》
CAS
2024年第1期123-125,共3页
Journal of Modern Laboratory Medicine
基金
深圳市医学三名工程(项目编号:SZSM201811092):输血技术学
深圳市医学重点学科(SZXK070):免疫遗传与输血技术学
广西医疗卫生重点培育学科[平南县人民医院消化内科2022(二):10]:消化系统疾病诊疗。
