摘要
目的检测慢性乙型肝炎患者肝组织中细胞间黏附分子-1(ICAM-1)和核因子(NF)-κB表达,分析其与肝脏炎症活动度和纤维化程度的关系。方法选取66例慢性乙型肝炎患者和10例非肝病对照者的肝活检标本,免疫组织化学和原位杂交法检测不同肝组织中ICAM-1、NF-κB蛋白表达情况。结果慢性乙型肝炎患者肝组织中ICAM-1、NF-κB蛋白阳性表达率高于正常肝组织,差异有统计学意义(P<0.05)。慢性乙型肝炎患者肝组织中ICAM-1、NF-κB蛋白表达与炎症活动度和纤维化程度呈正相关(r=0.493、0.496,P<0.01;r=0.580、0.519,P<0.01)。结论ICAM-1、NF-κB在慢性乙型肝炎患者中高表达,其有助于判断肝脏炎症活动度和纤维化程度。
Objective To detect the expressions of intercellular adhesion molecule-1(ICAM-1)and nuclear factor(NF)-κB in hepatic tissues of the patients with chronic hepatitis B,and to analyze their correlation with the hepatic inflammatory activity and fibrosis degree.Methods The liver biopsy specimens from 66 patients with hepatitis B and 10 non-hepatopathic controls were selected,and immunohistochemistry and in situ hybridization were used to detect ICAM-1 and NF-κB expression levels in different liver tissues.Results The positive rate of ICAM-1 and NF-κB expression in liver tissues of the patients with chronic hepatitis B was higher than that in normal liver tissues,and the difference was statistically significant(P<0.05).The expression of ICAM-1 and NF-κB in the patients with hepatitis B was positively correlated with the inflammatory activity and fibrosis degree(r=0.493,0.496,P<0.01;r=0.580,0.519,P<0.01).Conclusion ICAM-1 and NF-κB in the patients with chronic hepatitis B are highly expressed,which is useful in judging the hepatic inflammatory activity and fibrosis degree.
作者
李涛
李日林
谢宗恕
杨秀珍
佘东明
刘志娟
袁淑芳
LI Tao;LI Rilin;XIE Zongshu;YANG Xiuzhen;SHE Dongming;LIU Zhijuan;YUAN Shufang(Guilin Medicine University,Guilin,Guangxi 541004,China;Guangxi Medical University,Nanling,Guangxi 530021,China;Department of Infection,Liuzhou People’s Hospital,Liuzhou,Guangxi 545006,China)
出处
《重庆医学》
CAS
2024年第1期98-101,共4页
Chongqing medicine
基金
柳州市科学技术局基金项目(2020NBAB0821)。
关键词
细胞间黏附分子-1
核因子ΚB
慢性乙型肝炎
intercellular adhesion molecule-1
nuclear factor kappa-B
chronic hepatitis B
