摘要
目的研究miR-552、热休克蛋白90α(HSP90α)与喉癌临床病理因素及预后的相关性。方法选择2014年1月—2017年1月武汉市第三医院耳鼻喉科诊治的喉癌(LGC)患者87例作为LGC组,喉良性疾病患者45例为非LGC组,比较2组患者miR-552、HSP90α和临床病理因素差异;采用受试者工作特征(ROC)曲线分析miR-552和HSP90α预测喉癌患者死亡的效能;多因素Logistic回归分析喉癌患者死亡的危险因素。Kaplan-Meier分析miR-552和HSP90α与生存期的关系。结果LGC组患者miR-552和HSP90α表达水平显著高于非LGC组患者(t/P=11.076/<0.001、6.591/<0.001)。低分化、肿瘤最大径≥3 cm、有淋巴结转移及TNM分期Ⅲ+Ⅳ期喉癌患者miR-552和HSP90α表达显著高于中—高分化、肿瘤最大径<3 cm、无淋巴结转移及TNM分期Ⅰ+Ⅱ期患者(t/P=5.385/<0.001、11.480/<0.001、12.796/<0.001、7.562/<0.001、10.068/<0.001、10.776/<0.001、13.340/<0.001、9.769/<0.001);miR-552、HSP90α及二项联合预测喉癌患者死亡效能的AUC分别为0.812、0.806、0.925,二项联合的AUC最大(Z/P=4.218/0.009、4.416/0.007)。多因素Logistic回归分析显示miR-552≥1.7、HSP90α≥0.8、肿瘤分化程度低分化、肿瘤最大径≥3 cm、有淋巴结转移、TNM分期Ⅲ+Ⅳ期为喉癌死亡的独立危险因素[OR(95%CI)=2.557(1.027~5.182)、3.068(1.161~6.377)、1.962(1.035~5.729)、2.155(1.411~4.856)、3.442(1.306~5.713)、3.593(1.149~6.315)]。LGC组患者随访至终点时死亡64例,存活23例。miR-552≥1.7且HSP90α≥0.8的喉癌患者中位生存期为(31.6±5.4)月短于miR-552<1.7且HSP90α<0.8患者的(38.3±6.1)月(Log-rank=7.412,P=0.003)。结论喉癌患者miR-552和HSP90α表达显著升高,与临床病理因素及预后密切相关,可作为喉癌病情及预后评估的标志物。
Objective To study the correlation between miR-552,heat shock protein 90α(HSP90α)and clinicopathological factors and prognosis of laryngeal carcinoma.Methods From January 2014 to January 2017,87 patients with laryngeal cancer from the Department of Otorhinolaryngology of Wuhan Third Hospital were selected as the LGC group,and 45 patients with benign laryngeal diseases were selected as the non-LGC group.The miR-552,HSP90αand clinical and pathological factors were compared between the two groups;Receiver operating characteristic(ROC)was used to analyze the efficacy of miR-552 and HSP90αin predicting the death of patients with laryngeal cancer;Multivariate Logistic regression analysis was used to analyze the risk factors of death in patients with laryngeal cancer.Kaplan-Meier analysis of the relationship between miR-552 and HSP90αand survival.Results LGC group patients miR-552 and HSP90αThe expression was significantly higher in non LGC patients(t/P=11.076/<0.001,6.591/<0.001).MiR-552 and HSP90 in patients with poorly differentiated,maximum tumor diameter≥3cm,lymph node metastasis,and TNM stage III+IV laryngeal cancerαThe expression was significantly higher in patients with medium to high differentiation,maximum tumor diameter<3cm,no lymph node metastasis,and TNM stage I+II(t/P=5.385/<0.001,11.480/<0.001,12.796/<0.001,7.562/<0.001,10.068/<0.001,10.776/<0.001,13.340/<0.001,9.769/<0.001);MiR-552,HSP90αThe AUC for predicting the mortality efficacy of laryngeal cancer patients with binomial combination was 0.812,0.806,and 0.925,respectively.The AUC for binomial combination was the highest(Z/P=4.218/0.009,4.416/0.007).Multivariate logistic regression analysis showed that miR-552≥1.7 and HSP90α≥0.8,poorly differentiated tumor,maximum tumor diameter≥3cm,presence of lymph node metastasis,TNM stage III+IV are independent risk factors for laryngeal cancer death[OR(95%CI)=2.557(1.027-5.182),3.068(1.161-6.377),1.962(1.035-5.729),2.155(1.411-4.856),3.442(1.306-5.713),3.593(1.149-6.315)].64 patients in the LGC group died and 23 survived at the follow-up endpoint.MiR-552≥1.7 and HSP90α≥The median survival time of 0.8 laryngeal cancer patients is(31.6±5.4)months,shorter than miR-552<1.7 and HSP90α<0.8)Patient's(38.3±6.1)months(Log rank=7.412,P=0.003).Conclusions Laryngeal cancer patients miR-552 and HSP90αThe significantly increased expression is closely related to clinical pathological factors and prognosis,and can be used as a marker for evaluating the condition and prognosis of laryngeal cancer.
作者
曾妮
高芳芳
王爱华
陈应超
卢岭
李鹏程
Zeng Ni;Gao Fangfang;Wang Aihua;Chen Yingchao;Lu Ling;Li Pengcheng(Department of Otorhinolaryngology,Wuhan Third Hospital,Hubei Province,Wuhan 430074,China;不详)
出处
《疑难病杂志》
CAS
2024年第1期57-62,共6页
Chinese Journal of Difficult and Complicated Cases
基金
湖北省自然科学基金(2020HBA216)。
