摘要
旨在鉴定广西某鸽场分两批送检的疑似鸽副伤寒病例的病原并对病原菌的耐药性和毒力基因进行检测分析;对从病鸽肝脏组织分离的病原菌进行培养特性观察、生化鉴定、血清型鉴定及16S rRNA测序分析,并通过药敏试验、耐药基因和毒力基因检测对分离菌株进行耐药性和毒力分析。分离到2株革兰氏阴性短杆菌,结合生化鉴定、血清型鉴定和16S rRNA分析结果,表明2株分离菌株均为鼠伤寒沙门氏菌。药敏试验结果表明,2株鼠伤寒沙门氏菌均对萘啶酸、链霉素和四环素耐药,并呈现多重耐药表型,耐药基因检测结果与耐药表型相符。2株鼠伤寒沙门氏菌中invA等12种毒力基因检测呈阳性。结果表明,成功分离鉴定2株具有多重耐药性并携带大量毒力基因的鸽源鼠伤寒沙门氏菌,可为鸽源鼠伤寒沙门氏菌的防治和研究提供参考。
This research aimed to identify the pathogens of two batches of suspected pigeon paratyphoid infections in a pigeon farm in Guangxi and to analyze the drug resistance and virulence genes of the pathogenic bacteria.A series of tests,including pathological dissection,bacterial isolation and culture,observation of culture characteristics,biochemical identification,16S rRNA sequencing analysis,and serotype analysis were performed on liver tissues of diseased pigeons,and drug resistance and virulence analysis were analyzed by drug sensitivity test,drug resistance gene and virulence gene detection.Two short Gram-negative bacilli were isolated from the liver tissue of diseased pigeons.Combined with the results of biochemical identification,serotype identification and 16S rRNA analysis,the two strains were all Salmonella typhimurium.The results of the drug sensitivity test showed that both strains of Salmonella typhimurium were resistant to nalidixic acid,streptomycin,and tetracycline.Still,both showed multiple drug-resistance phenotypes.The results of drug resistance gene detection were consistent with the drug resistance phenotypes.The isolated bacterial strains tested positive for 12 virulence genes,including invA.In this study,two strains of Salmonella typhimurium were successfully isolated and identified from pigeons,which showed multiple drug resistance and had high virulence,and provided a reference for the prevention and research of Salmonella typhimurium infection from pigeons in the subsequent work.
作者
陆凡
王鹏
刘林敏
黄振兴
张明霞
杜文珍
吴颖臻
韦平
韦天超
LU Fan;WANG Peng;LIU Lin-min;HUANG Zhen-xing;ZHANG Ming-xia;DU Wen-zhen;WU Ying-zhen;WEI Ping;WEI Tian-chao(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi,530004,China;Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics,Nanning,Guangxi,530004,China)
出处
《动物医学进展》
北大核心
2024年第1期36-43,共8页
Progress In Veterinary Medicine
基金
广西重点研发计划项目(桂AB23026082)
广西科技重大专项项目(桂科AA17204057)
广西大学“大学生创新创业训练计划”项目(S202210593218)
校企合作项目(202201277)。
关键词
鸽
鼠伤寒沙门氏菌
分离鉴定
多重耐药性
毒力基因
pigeon
Salmonella typhimurium
isolation and identification
multidrug resistance
virulence gene
