摘要
体外核酸扩增技术在动物疫病病原检测中应用广泛。重组酶聚合酶扩增(RPA)作为一种新型的核酸等温扩增技术,可在较低温度(37~42℃)下扩增DNA或RNA,具有反应时间短、样品制备量少、特异性和敏感性高等优点。RPA突破了常规聚合酶链反应(PCR)实施时对实验室条件的依赖性,并能高效和灵敏地检测动物疫病病原。基于RPA技术的病原菌检测方法可实现对动物细菌病的现场快速诊断。论文综述了RPA技术的反应体系和条件、反应原理和产物检测方法等,介绍了RPA技术在动物病原菌检测中的应用和潜在价值,以期为动物疾病快速诊断研究提供参考。
Technology of nucleic acid amplification in vitro is widely used in animal epidemic disease detection.As a new isothermal amplification technique,RPA can amplify DNA or RNA at a constant low temperature(37-42℃),with the advantages of short reaction time,minimal sample preparation,high specificity and sensitivity.Recombinase polymerase amplification technology(RPA)breaks through the dependence of traditional polymerase chain reaction(PCR)on laboratory conditions,and it is becoming a method for the effective and sensitive detection of pathogens of animal epidemics.The pathogen detection method based on RPA technology can achieve rapid on-site diagnosis of animal bacterial diseases.In this paper,the composition of reaction system,the reaction conditions,the reaction principle,and the detection methods of amplified products of RPA were introduced,and the application and potential value of RPA in the detection of animal bacterial diseases were also introduced,aiming at providing the reference for the rapid diagnosis of animal diseases.
作者
张蕾
陈亮
江波涛
冯万宇
兰世捷
苗艳
沈思思
李丹
田秋丰
杨昊天
张艳
刘雪松
黄宣凯
钟鹏
史同瑞
ZHANG Lei;CHEN Liang;JIANG Bo-tao;FENG Wan-yu;LAN Shi-jie;MIAO Yan;SHEN Si-si;LI Dan;TIAN Qiu-feng;YANG Hao-tian;ZHNAG Yan;LIU Xue-song;HUANG Xuan-kai;ZHONG Peng;SHI Tong-rui(Branch of Animal Husbandry and Veterinary,Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang,161000,China)
出处
《动物医学进展》
北大核心
2024年第1期89-94,共6页
Progress In Veterinary Medicine
基金
黑龙江省农业科学院畜牧兽医分院自拟课题(ZNKT-202212)
齐齐哈尔市科技计划创新激励项目(CNYGG-2023019,CNYGG-2022004)。
关键词
重组酶聚合酶扩增
快速检测
病原菌
应用
recombinase polymerase amplification
rapid detection
pathogenic bacteria
application
