摘要
利用体外细胞模型探究乳酸菌/水仙鳞茎发酵滤液的活性,开发其潜在功效。将乳酸菌接种至水仙鳞茎水提物中,发酵培养72 h,收集制备乳酸菌/水仙鳞茎发酵滤液,通过CCK实验探究其细胞毒性,利用体外HaCaT细胞保湿模型、干燥模型、RAW264.7细胞抗炎模型和透明质酸酶抑制抗敏模型,探究乳酸菌/水仙鳞茎发酵滤液的保湿和舒缓功效及其原理。结果显示,乳酸菌/水仙鳞茎发酵滤液可以有效促进HaCaT细胞中AQP3的产生与表达,促进FLG的表达,从而实现保湿功效;也可以通过抑制透明质酸酶的活力、抑制RAW264.7细胞释放炎症介质NO、TNF-α和IL-6,从而实现抗敏抗炎的舒缓功效。乳酸菌/水仙鳞茎发酵滤液可以作为保湿舒缓化妆品原料之一。
The activity of lactic acid bacteria/narcissus bulb fermentation filtrate was studied by cell model in vitro to explore its potential efficacy.Lactic acid bacteria were inoculated into the water extract of narcissus bulbs and fermented for 72 h.The lactic acid bacteria/narcissus bulb fermentation filtrate was collected.The cytotoxicity of the fermentation filtrate was tested by CCK.The moisturizing and soothing effects of the fermentation filtrate,as well as the mechanism thereof,were studied by using the moisturizing model and the drying model of HaCaT in vitro,the anti-inflammatory model of RAW264.7,and the anti-allergic model of hyaluronidase inhibition.The results showed that,the lactic acid bacteria/narcissus bulb fermentation filtrate could effectively promote the production and expression of AQP3 in HaCaT cells,and promote the expression of FLG,so as to achieve moisturizing effect.It could also achieve anti-allergic and anti-inflammatory(soothing)effects by inhibiting the activity of hyaluronidase and inhibiting the release of inflammatory mediators(NO,TNF-α and IL-6)from RAW264.7 cells.This work showed that lactic acid bacteria/narcissus bulb fermentation filtrate could be used as one of the raw materials for moisturizing and soothing cosmetics.
作者
潘发伍
PAN Fa-wu(Juwenlee(FuJian)Cosmetics Co.,Ltd.,Zhangzhou,Fujian 363107,China)
出处
《日用化学品科学》
2024年第1期22-28,共7页
Detergent & Cosmetics
关键词
水仙鳞茎
乳酸菌
保湿
舒缓
narcissus bulb
lactic acid bacteria
moisturizing
soothing
