摘要
为探究接种丛枝菌根真菌(AMF)促进毛红椿幼苗抗旱性机制,以毛红椿幼苗为材料,探究接种AMF对毛红椿幼苗在正常浇水(NW)、轻度干旱胁迫(LS)、中度干旱胁迫(MS)和重度干旱胁迫(SS)处理下的生长和光合生理影响。结果表明:在干旱胁迫下,毛红椿幼苗生长和生理均受到不同程度抑制,但接种AMF可缓解干旱胁迫对苗木生长的抑制,显著促进毛红椿幼苗苗高和地径的生长(P<0.05)。接种AMF与对照(CK)的毛红椿幼苗叶片除气孔导度(G_(s))在中度干旱胁迫下差异不显著外,G_(s)在另外3个干旱胁迫处理下及蒸腾速率(T_(r))、胞间二氧化碳浓度(C_(i))、净光合速率(P_(n))在4个干旱胁迫处理下均达到显著差异(P<0.05)。在重度干旱胁迫下,接种AMF毛红椿幼苗叶片的脯氨酸(Pro)含量较CK提高了26.45%,其可溶性蛋白(Sp)含量与CK存在显著差异(P<0.05)。干旱胁迫下,与CK相比,接种AMF毛红椿幼苗叶片的丙二醛(MDA)含量较低,而超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性较高。由相关性分析可知,接种幼苗的内含物(Ss、Sp、Pro)与抗氧化酶(SOD、POD、CAT)活性之间均存在极显著正相关(P<0.01,P<0.001),光合指标除C_(i)外,其余光合指标(P_(n)、G_(s)、T_(r))与生长指标(GH、GD)均存在正相关。接种AMF能够通过提高毛红椿幼苗叶片的光合能力及渗透调节能力以增强其抗旱性,促进毛红椿幼苗生长,有效提高毛红椿幼苗对干旱胁迫的适应性。
In order to explore the mechanism of inoculation of arbuscular mycorrhizal fungi(AMF)to promote the drought resistance of Toona ciliata var.pubescens seedlings,the effects of AMF inoculation on the growth and photosynthetic physiology of T.ciliata var.pubescens seedlings under normal watering(NW),mild drought stress(LS),moderate drought stress(MS)and severe drought stress(SS)were investigated.The results showed that the growth and physiology of T.ciliata var.pubescens seedlings were inhibited to varying degrees under drought stress,but AMF inoculation could alleviate the inhibition of drought stress on seedling growth and significantly promote the growth of seedling height and ground diameter of T.ciliata var.pubescens seedlings(P<0.05).In addition to stomatal conductance(G_(s)),there was no significant difference between AMF and CK in the leaves of T.ciliata var.pubescens seedlings under MS,transpiration rate(T_(r)),intracellular CO 2 concentration(C_(i)),G_(s) and net photosynthetic rate(P_(n))reached significant difference levels under four drought stress levels(P<0.05).Under SS,the content of proline(Pro)in the inoculated group increased by 26.45%compared with CK.The content of soluble protein(Sp)in the leaves of AMF and CK seedlings was significantly different(P<0.05).Under drought stress,compared with CK,the content of malondialdehyde(MDA)in the leaves of T.ciliata var.pubescens seedlings inoculated with AMF was lower,while the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)were higher.Correlation analysis showed that there was a highly significant positive correlation between the contents of inoculated(Ss,Sp,Pro)seedlings and the activity of SOD,POD and CAT(P<0.01,P<0.001),and there was positive correlation between the photosynthetic indexes(P_(n),G_(s) and T_(r))and the growth indexes(GH,GD)except the C_(i).Inoculation with AMF can enhance the drought resistance of T.ciliata var.pubescens seedlings by improving their photosynthetic capacity and osmotic adjustment ability,promote the growth of T.ciliata var.pubescens seedlings,and effectively improve the adaptability of T.ciliata var.pubescens seedlings to drought stress.
作者
杜宣瑾
朱源
张露
贾婷
刘玮
马际凯
DU Xuanjin;ZHU Yuan;ZHANG Lu;JIA Ting;LIU Wei;MA Jikai(Jiangxi Provincial Key Laboratory of Silviculture,Nanchang,Jiangxi 330045,China;College of Forestry,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China)
出处
《森林与环境学报》
CSCD
北大核心
2024年第1期71-78,共8页
Journal of Forest and Environment
基金
国家自然科学基金项目“毛红椿根蘗性状发生的生理和遗传机制”(31860202)
国家重点研发专项子课题“毛红椿、木荷高效培育技术研究”(2016YFD060060605)。
关键词
毛红椿
干旱胁迫
丛枝菌根真菌
生长
生理
Toona ciliata var.pubescens
drought stress
arbuscular mycorrhizal fungi
growth
physiology
