摘要
背景:胰岛素抵抗与小肠黏膜通透性的关系可能与脂肪过多、炎症和氧化应激等有关。目前不同种类的运动对该关系的影响尚不清楚,还需要进一步进行相关机制研究。目的:以高脂饲料诱导胰岛素抵抗小鼠模型,观察不同形式的长期运动(持续运动和累积运动)对胰岛素抵抗小鼠肠黏膜通透性的影响,并比较不同运动强度的效果差异,以评价累积运动的健康促进效应。方法:采用4周龄雄性C57BL/6J小鼠,以高脂饲料诱导胰岛素抵抗小鼠模型,造模成功的小鼠随机分为5组,分别为高脂胰岛素抵抗组、普食胰岛素抵抗组、中强度持续运动组、中强度累积运动组、高强度累积运动组,其中高脂胰岛素抵抗组饲以高脂饲料,其余各组饲以普通饲料。各运动组接受8周的不同形式的跑台训练:中强度持续运动组小鼠进行50 min,速度为11 m/min的运动;中强度累积运动组小鼠进行每天4次,每次12.5 min(次与次间隔3 h),速度为11 m/min的运动;高强度累积运动组小鼠进行每天4次、每次7.5 min(次与次间隔3 h),速度为19 m/min的运动。末次运动48 h后取材,检测各组小鼠血清中的脂多糖和右旋乳酸含量,采用苏木精-伊红染色观察回肠组织病理变化,通过Western blot法检测肠道紧密连接蛋白的表达,ELISA法测定血液和回肠组织中的炎症因子白细胞介素10、肿瘤坏死因子α及肠道黏膜分泌性免疫球蛋白A的表达。结果与结论:①高脂膳食诱导的胰岛素抵抗小鼠伴有体质量增加、血清内毒素和右旋乳酸水平显著升高、血清和小肠组织促炎因子肿瘤坏死因子α水平显著增加等表现;长期规律性的累积运动和持续运动均可降低胰岛素抵抗小鼠的体质量,并明显改善糖代谢功能,纠正或改善胰岛素抵抗症状;②长期规律性的累积运动和持续运动均可提高肠黏膜组织中紧密连接蛋白的表达量并增加分泌性免疫球蛋白A分泌量,从而改善肠黏膜通透性,增强肠道免疫功能,降低血清中内毒素脂多糖的含量,进而降低循环血和肠组织中的促炎症因子表达,最终发挥对胰岛素抵抗小鼠肠黏膜屏障的保护作用;③等运动量的高强度累积运动与中强度累积运动、中强度持续运动相比,在降低胰岛素抵抗小鼠的体质量、改善胰岛素抵抗症状、保护肠黏膜屏障方面效果更明显。
BACKGROUND:The relationship between insulin resistance and intestinal mucosal permeability may be related to excess fat,inflammation and oxidative stress.At present,the influence of different kinds of exercise on this relationship has not been fully studied,and the relevant mechanism needs to be further studied.OBJECTIVE:To study the effects of continuous exercise and cumulative exercise on intestinal mucosal permeability of insulin-resistant mice induced by high fat diet,and to compare the effects of different intensities of exercise,thereby evaluating the health-promoting effects of cumulative exercise.METHODS:Four-week-old male C57BL/6J mice were fed high-fat diet to induce insulin resistance,and mice with successful modeling were randomly divided into five groups:high-fat insulin resistance group,general dietary insulin resistance group,moderate-intensity continuous exercise group,moderate-intensity cumulative exercise group,and high-intensity cumulative exercise group.Mice in the high-fat insulin resistance group received high-fat diet and mice in the other groups were fed normally.All the exercise groups received 8 weeks of different forms of treadmill training.Mice in the moderate-intensity sustained exercise group exercised for 50 minutes at a speed of 11 m/min.Mice in the moderate-intensity cumulative exercise group were subjected to 12.5 minutes of exercise,four times a day(3 hours between sessions),at a speed of 11 m/min.Mice in the high-intensity cumulative exercise group exercised for 7.5 minutes once,four times a day(3 hours between sessions),at a speed of 19 m/min.At 48 hours after the final session,the levels of lipopolysaccharide and D-lactic acid in serum of mice were detected,the pathological changes of ileal tissue were observed by hematoxylin-eosin staining,and the expression of intestinal compact linking protein was detected by western blot.The expression of interleukin-10,tumor necrosis factor α in blood and ileum and intestinal secreted immunoglobulin A were detected by ELISA.RESULTS AND CONCLUSION:High-fat diet-induced insulin resistance was associated with body mass gain.The serum levels of endotoxin and D-lactic acid significantly increased,and the levels of tumor necrosis factor-αin serum and small intestine were significantly increased.Long-term regular cumulative exercise and continuous exercise could reduce the body mass of insulin-resistant mice,significantly improve glucose metabolism,and correct or improve insulin resistance symptoms.Both long-term regular cumulative exercise and continuous exercise could increase the expression of zonula occludens protein 1 and increase the secretion of secreted immunoglobulin A in intestinal mucosal tissue,thereby improving intestinal mucosal permeability,enhancing intestinal immune function,reducing the content of lipopolysaccharide in serum,and reducing the expression of pro-inflammatory factors in circulating blood and intestinal tissue.Finally,it could protect the intestinal mucosal barrier of insulin-resistant mice.Compared with medium-intensity cumulative exercise and continuous exercise,high-intensity cumulative exercise had more obvious effects on reducing body mass,improving insulin resistance symptoms and protecting intestinal mucosal barrier in insulin-resistant mice.
作者
梁飞
Liang Fei(School of Physical Education,Shaoguan 512005,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
2024年第27期4332-4339,共8页
Chinese Journal of Tissue Engineering Research
基金
2021年度广东省普通高校特色创新项目(2021WTSCX087),项目负责人:梁飞
2021年度广东省高等教育改革项目(202129),项目负责人:梁飞
韶关学院2020年梁飞博士科研启动基金项目,项目负责人:梁飞。
关键词
持续运动
累积运动
胰岛素抵抗
小鼠
肠黏膜
通透性
屏障功能
炎症
continuous exercise
cumulative exercise
insulin resistance
mouse
intestinal mucosa
permeability
barrier function
inflammation
