摘要
【目的】了解广西鸭圆环病毒(Duck circovirus,DuCV)的遗传进化特征。【方法】试验于2019—2022年从广西地区采集761份病死鸭临床样本,利用实时荧光定量PCR检测DuCV感染情况。通过PCR扩增DuCV全基因组序列,利用DNAStar分析其与参考毒株核苷酸和氨基酸序列相似性,使用RDP4和SimPlot 3.5.1软件分析DuCV重组事件,采用Mega 7.0软件构建遗传进化树,应用BioEdit软件分析DuCV Cap蛋白氨基酸变异位点。【结果】DuCV阳性率为22.5%,基因组大小为1988~1996 bp。广西DuCV毒株全基因组、Rep和Cap基因与参考毒株核苷酸序列相似性分别为71.9%~99.6%、83.1%~99.9%和52.8%~99.9%。基因重组分析显示,DuCV-GX13-2019和DuCV-GX14-2020存在重组事件。Cap蛋白共有78个氨基酸位点发生突变,与DuCV-1相比,DuCV-2在第3、12、15、23、31、42、56和64位氨基酸处完全变异。广西毒株为DuCV-1和DuCV-2基因型,DuCV-1为主要优势毒株,且DuCV-1b亚型流行最为广泛。【结论】广西地区DuCV发病较严重,部分毒株存在重组事件,Cap蛋白突变率高,DuCV多种亚型均有流行。研究结果为广西地区DuCV流行病学调查提供基本数据。
【Objective】The aim of this experiment was to understand the genetic and evolutionary characteristics of Duck circovirus(DuCV)in Guangxi.【Method】A total of 761 clinical samples of ducks were collected from Guangxi during 2019-2022 to detect DuCV infection by Real-time quantitative PCR.The whole genome sequence of DuCV was amplified by PCR,the similarity of nucleotide and amino acid sequences between DuCV and reference strain were analyzed by DNAStar,the DUCV recombination event was analyzed by RDP4 and SimPlot 3.5.1 software,and the genetic evolution tree was constructed by Mega 7.0 software.The amino acid variation sites of DuCV Cap protein were analyzed by BioEdit software.【Result】The positive rate of DuCV was 22.5%,and the genome size was between 1988-1996 bp.The nucleotide sequence similarity of the whole genome,Rep and Cap genes between the Guangxi DuCV strain and the reference strain was 71.9%-99.6%,83.1%-99.9%and 52.8%-99.9%,respectively.Gene recombination analysis showed that there were recombination events in DuCV-GX13-2019 and DuCV-GX14-2020.A total of 78 amino acid sites in Cap protein were mutated.Compared with DuCV-1,DuCV-2 was completely mutated at amino acids 3,12,15,23,31,42,56 and 64.The strains in Guangxi were DuCV-1 and DuCV-2 genotypes,DuCV-1 was the main dominant strain,and DuCV-1b subtype was the most widely prevalent.【Conclusion】The incidence of DuCV infection in Guangxi was serious,some strains had recombination events,the mutation rate of Cap protein was high,and various subtypes of DuCV were prevalent,which provided basic data for the epidemiological investigation of DuCV in Guangxi.
作者
熊陈勇
曾素先
刘惠心
赵康
施开创
施喻文
谢守玉
韦显凯
龙凤
冯淑萍
屈素洁
王露霞
林昌华
尹彦文
XIONG Chenyong;ZENG Suxian;LIU Huixin;ZHAO Kang;SHI Kaichuang;SHI Yuwen;XIE Shouyu;WEI Xiankai;LONG Feng;FENG Shuping;QU Sujie;WANG Luxia;LIN Changhua;YIN Yanwen(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Shajing Subdistrict Office Jiangnan District,Nanning City Aquatic Animal Husbandry and Veterinary Station,Nanning 530031,China;Guangxi State Farms Yongxin Animal Husbandry Group Xijiang Co.,Ltd.,Guigang 537104,China;Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China;Baise Center for Animal Disease Control and Prevention,Baise 533000,China)
出处
《中国畜牧兽医》
CSCD
北大核心
2024年第1期373-381,共9页
China Animal Husbandry & Veterinary Medicine
基金
广西自然科学基金青年科学基金项目(2020GXNSFBA297066)
广西农业科技资助项目(Z201984、Z202032)。
