摘要
目的比较大黄-丹参药对在不同配伍比例时两药7个有效成分(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、丹酚酸B、丹参酮ⅡA)溶出率和抗模型大鼠肾纤维化作用的差异。方法采用高效液相色谱(HPLC)法测定大黄单提液、丹参单提液及6种配伍比例(1∶1、1∶1.25、1∶1.75、1∶2、1∶3、1∶4)大黄-丹参混提液中7个有效成分的含量及溶出率。采用单侧输尿管梗阻复制肾纤维化大鼠模型,比较大黄、丹参及1∶1.25、1∶1.75、1∶2配伍比例大黄-丹参混提液对模型大鼠肾组织病理形态及血尿素氮(BUN)、血清肌酐(SCr)、24 h尿蛋白(24 h-UTP)水平的影响。结果不同配伍比例大黄-丹参混提液中7个有效成分含量均小于单提液,大黄-丹参(1∶2)混提液中有效成分整体溶出率优于其他配伍比例。与模型组比较,大黄单提液、丹参单提液及1∶1.25、1∶1.75、1∶2配伍比例大黄-丹参混提液作用下,大鼠肾纤维化程度显著减轻,BUN、SCr、24 h-UTP水平均显著降低,其中以大黄单提液作用最强。结论所建立的含量测定方法可用于大黄-丹参药对中7个有效成分的含量测定;大黄-丹参不同比例配伍后有效成分溶出率均小于单提液;大黄-丹参配伍后在抗模型大鼠肾纤维化作用方面未产生协同作用,大黄单提液抗肾纤维化作用最佳。
Objective To compare Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma drug pair with different compatibility proportions on the dissolution rate of seven active ingredients(aloe-emodin,rhein,emodin,chrysophanol,physcion,salvianolic acid B and tanshinoneⅡA)and anti-renal fibrosis in rats.Methods High-performance liquid chromatography(HPLC)method was used to determine the content and dissolution rate of seven active ingredients in single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with six compatibility proportions(1∶1,1∶1.25,1∶1.75,1∶2,1∶3 and 1∶4).Rat models of renal fibrosis were replicated by unilateral ureteral obstruction.The effects of single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with three compatibility proportions(1∶1.25,1∶1.75 and 1∶2)on the pathological morphology of renal tissue,blood urea nitrogen(BUN),serum creatinine(SCr)and 24-hour urine total protein(24 h-UTP)levels in rats were compared.Results The content of seven active ingredients in the mixed extract of Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma with different compatibility proportions was lower than that in the above two single extract.The overall dissolution rate of active ingredients in the mixed extract of drug pair at a compatibility proportion of 1∶2 was better than that at other compatibility proportions.Compared with that in the model group,the degree of renal fibrosis in rats significantly decreased under the single extract of Rhei Radix et Rhizoma,Salviae Miltiorrhizae Radix et Rhizoma and mixed extract of drug pair with three compatibility proportions(1∶1.25,1∶1.75 and 1∶2),and the BUN,SCr and 24 h-UTP levels significantly decreased,with the most obvious improvement under the extract of Rhei Radix et Rhizoma.Conclusion The established method can be used for the content determination of seven active ingredients in Rhei Radix et Rhizoma-Salviae Miltiorrhizae Radix et Rhizoma drug pair.The dissolution rate of active ingredients in the drug pair with different compatibility proportions is lower than that in the two single extract.The compatibility of Rhei Radix et Rhizoma and Salviae Miltiorrhizae Radix et Rhizoma do not have a synergistic effect on the anti-renal fibrosis,and the extract of Rhei Radix et Rhizoma is the most effective.
作者
孟颖
徐子寒
高俪原
韩怡
MENG Ying;XU Zihan;GAO Liyuan;HAN Yi(Zhangjiagang Hospital of Traditional Chinese Medicine,Zhangjiagang,Jiangsu,China 215600)
出处
《中国药业》
CAS
2024年第2期37-42,共6页
China Pharmaceuticals
基金
江苏省药学会—奥赛康医院药学基金[A202126]
江苏省药学会—天晴医院药学基金[Q202125]
江苏省苏州市科技发展计划指导性项目[SKYD2023228,SKYXD2022066]
江苏省苏州市科技发展计划(民生科技-医疗卫生应用基础研究)项目[SYSD2020221]
江苏省张家港市科技计划项目[ZKS2135,ZKS2147]
江苏省张家港市卫生青年科技项目[ZJGQNKJ202235]。
关键词
大黄
丹参
配伍比例
溶出率
肾纤维化
大鼠
Rhei Radix et Rhizoma
Salviae Miltiorrhizae Radix et Rhizoma
compatibility proportion
dissolution rate
renal fibrosis
rat
