摘要
建立南五味子药材超高效液相色谱(UPLC)指纹图谱和6种木脂素类成分一测多评法(QAMS),为南五味子药材质量标准提高提供参考。采用UPLC法建立南五味子药材指纹图谱,运用聚类分析(HCA)和正交偏最小二乘法-判别分析(OPLS-DA)对指纹图谱进行分析;以五味子酯甲为内参物,建立五味子酯丙、五味子酯乙、安五脂素、五味子甲素和五味子酯丁的一测多评法,并与外标法(ESM)测定结果对比,判断方法的准确性。14批南五味子药材指纹图谱确定了15个共有峰,通过对照品比对指认了其中7个成分,分别为原儿茶酸、五味子酯丙、五味子酯甲、五味子酯乙、安五脂素、五味子甲素、五味子酯丁,其指纹图谱相似度均在0.95以上;通过聚类分析(HCA)和正交偏最小二乘法-判别式分析(OPLS-DA)可以将南五味子药材分为4类,峰1(原儿茶酸)、峰2、峰4、峰6、峰7(五味子酯乙)、峰8、峰9、峰12、峰15共9个成分是导致产地差异性的主要标志物。一测多评法与外标法测定的五味子酯丙、五味子酯乙、安五脂素、五味子甲素和五味子酯丁含量结果无显著差异。所建立的方法简便、可靠,为南五味子药材质量评价提供参考。
To provide a reference for improving the quality standard of Schisandrae Sphenantherae Fructus,the ultra performance liquid chromatography(UPLC)fingerprint of Schisandrae Sphenantherae Fructus and the contents of six lignans by a method of quantitative analysis of multi-components by single marker(QAMS)were established.The fingerprints of Schisandrae Sphenantherae Fructus were established by UPLC and the UPLC fingerprints were analyzed by hierarchical cluster analysis(HCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)with schisantherin A as the internal reference,QAMS was established for the determinations of schisantherin C,schisantherin B,anwuligan,schisandrin A and schisantherin D,and compared with the results of external standard method(ESM)to determine the accuracy of the method.Fifteen common peaks were marked in the fingerprints of 14 batches of Schisandrae Sphenantherae Fructus,seven of which were identified by comparison with reference substance,including protocatechuic acid,schisantherin C,schisantherin A,schisantherin B,anwuligan,schisandrin A,schisantherin D.The fingerprint similarities were above 0.95.Schisandrae Sphenantherae Fructus can be divided into four categories by HCA and OPLS-DA.The nine components,including peak 1(protocatechuic acid),peak 2,peak 4,peak 6,peak 7(schisantherin B),peak 8,peak 9,peak 12,and peak 15,were the main makers of the difference in origin.There was no significant difference between the contents of schisantherin C,schisantherin B,anwuligan,schisantherin A,and schisantherin B determined by QAMS and ESM.The method is simple and reliable,providing a reference for the quality evaluation of Schisandrae Sphenantherae Fructus.
作者
刘晓霞
位翠杰
冯涌微
张芳平
段志文
谢文凯
何民友
李振雨
LIU Xiao-xia;WEI Cui-jie;FENG Yong-wei;ZHANG Fang-ping;DUAN Zhi-wen;XIE Wen-kai;HE Min-you;LI Zhen-yu(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第1期1-12,共12页
Natural Product Research and Development
基金
国家工信部2022年产业技术基础公共服务平台项目(2022-230-221)。
关键词
南五味子
指纹图谱
含量测定
一测多评
质量评价
Schisandrae Sphenantherae Fructus
fingerprint
content determination
QAMS
quality evaluation
