摘要
研究黄姜花花提取物的化学成分、抗氧化、酶抑制活性及抗炎作用。本文采用回流提取法制得黄姜花花水提物和醇提物,使用超高效液相色谱-四极杆静电场轨道阱串联质谱(UHPLC-Q-Orbitrap MS)分析其提取物化学成分,共鉴定出58种化合物,包括11种酚类和18种黄酮类化合物。黄姜花花提取物具有较好的抗氧化活性,对DPPH、ABTS自由基的清除作用较强,同时能够抑制α-葡萄糖苷酶、酪氨酸酶、乙酰胆碱酯酶和丁酰胆碱酯酶的活性。提取物在无毒范围内(62.5、125、250μg/mL)能显著降低LPS诱导的RAW 264.7细胞中促炎症介质(NO和PGE 2)和细胞因子(TNF-α、IL-6和IL-1β)的过度分泌。本实验为黄姜花花在食品、药品、化妆品等行业的开发利用提供理论基础。
This study aimed to investigate the chemical composition,antioxidant,enzyme inhibitory,and anti-inflammatory activities of Hedychium flavum flower extracts.H.flavum flower water extracts and ethanol extracts were obtained by reflux extraction method.The chemical composition of the extracts was identified by ultra-high-performance liquid chromatography coupled to quadrupole-orbitrap mass spectrometry(UHPLC-Q-Orbitrap-MS).A total of 58 compounds were identified,including 11 phenols and 18 flavonoids.The extracts showed strong antioxidant activity in the DPPH and ABTS assay and had certain inhibitory effects onα-glucosidase,tyrosinase,acetylcholinesterase,and butyrylcholinesterase.Within the non-toxic dose range(62.5,125,250μg/mL),the extracts significantly reduced LPS-induced oversecretion of pro-inflammatory mediators(NO and PGE 2)and cytokines(TNF-α,IL-6,IL-1β)in RAW 264.7 cells.Our study provides a theoretical basis for the development and utilization of H.flavum flower in the food,pharmaceutical,and cosmetic industries.
作者
丁芙蓉
贾小演
吴相欢
吴霞
杨念
刘雄利
田民义
DING Fu-rong;JIA Xiao-yan;WU Xiang-huan;WU Xia;YANG Nian;LIU Xiong-li;TIAN Min-yi(School of Liquor and Food Engineering,Guizhou University;College of Life Sciences,Guizhou University;National&Local Joint Engineering Research Center for the Exploitation of Homology Resources of Southwest Medicine and Food,Guiyang 550025,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2024年第1期13-25,共13页
Natural Product Research and Development
基金
国家自然科学基金(82360834)。
关键词
黄姜花
提取物
化学成分
抗氧化活性
酶抑制活性
抗炎活性
Hedychium flavum
extracts
chemical composition
antioxidant activity
enzyme inhibition activity
anti-inflammatory activity
