摘要
目的:研究鱼鳔、驴皮化学成分及对环磷酰胺所致贫血小鼠的改善作用。方法:测定总蛋白含量、氨基酸组成及分子量范围;胃蛋白酶辅助提取获得胶原蛋白,并分析其含量、组成及结构;腹腔注射环磷酰胺(80 mg/kg)建立小鼠贫血模型,灌胃给予受试物(鱼鳔胶1.52 g/(kg·d)、驴皮胶1.39 g/(kg·d)),观察对脏器系数和血液学指标的影响。结果:鱼鳔、驴皮总蛋白含量均大于97%,鱼鳔胶、驴皮胶分子量在50 Da~328 kDa之间;鱼鳔、驴皮胶原蛋白均为Ⅰ型胶原蛋白,鱼鳔胶原蛋白含量约为驴皮的6.51倍,但胶原蛋白三螺旋结构不同,鱼鳔胶原蛋白以α-螺旋结构为主,驴皮胶原蛋白以反平行为主。功效评价结果表明,给药28 d,鱼鳔胶与驴皮胶均可促进环磷酰胺贫血小鼠造血机能的恢复,以鱼鳔胶效果更佳。与模型组相比,鱼鳔胶给药组红细胞、血红蛋白、血小板、网织红细胞、脾脏及胸腺系数均得到显著改善(P<0.05);驴皮胶给药组红细胞、血红蛋白及胸腺系数显著改善(P<0.05)。结论:对鱼鳔、驴皮化学成分进行系统研究,确定了化学成分间的差异;发现鱼鳔胶、驴皮胶均可促进环磷酰胺贫血小鼠造血机能的恢复,且鱼鳔胶效果更佳,推测二者补血活性可能与氨基酸、胶原蛋白等成分有关。本研究为鱼鳔资源精深加工奠定理论基础。
Objective:To systematic study for different chemical components of swim bladder and donkey skin,and their improvement effects on cyclophosphamide-induced anemia mice.Methods:The chemical characterizations,including total protein content,amino acid composition and molecular weight were measured,respectively.Pepsin-soluble collagens were extracted from swim bladder and donkey skin by hydrochloric acid-pepsin method,and analyzed the content,composition and structure.The anemia model was established by intraperitoneal injected with cyclophosphamide at a dosage of 80 mg/kg.The coefficient of visceral organs and the hematological indexes were observed in mice with swim bladder glue(1.52 g/(kg·d))or donkey skin glue(1.39 g/(kg·d))by gavage administration per day.Results:The total protein contents of swim bladder and donkey skin reached more than 97%.The molecular weights of swim bladder glue and donkey skin glue ranged from 50 Da to 328 kDa.Swim bladder collagen and donkey skin collagen were both typeⅠcollagen.While the content of collagen in swim bladder was 6.51 times than that in donkey skin.However the collagen triple helix structures were different.The swim bladder collagen had anα-helix structure,and the donkey skin collagen structure was mainly anti-parallel.After 28 days of administration,both swim bladder glue and donkey skin glue could promote the recovery of hematopoietic function in cyclophosphamide-induced anemia mice,and swim bladder glue exhibited a better effect.Compared with the model,the erythrocyte,hemoglobin,platelet,reticulocyte,spleen and thymus coefficients of the swim bladder glue were significantly improved(P<0.05).The erythrocyte,hemoglobin and thymus coefficients of the donkey skin glue were significantly improved(P<0.05).Conclusion:A systematic study of the chemical characteristics of swim bladder and donkey skin were carried out to determine the differences between the components.Pharmacodynamical research showed that swim bladder glue and donkey skin glue could promote the recovery of hematopoietic function in cyclophosphamide model mice,and the effect of swim bladder glue was better.It was hypothesized that the blood replenishing activity of the two might be related to the amino acids,collagen and other components.This work could provide a theoretical foundation for the development of swim bladder.
作者
孙皓岩
胡淑曼
魏宝红
马晓青
杨文哲
刘佳
杨雪
邱培菊
SUN Haoyan;HU Shuman;WEI Baohong;MA Xiaoqing;YANG Wenzhe;LIU Jia;YANG Xue;QIU Peiju(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;Marine Biomedical Research Institute of Qingdao,Qingdao 266071,China)
出处
《食品工业科技》
CAS
2024年第3期338-345,共8页
Science and Technology of Food Industry
基金
山东省重点研发计划(重大科技创新工程)(2021CXGC010510)
中央引导地方科技发展专项资金(YDZX2021018)。
关键词
鱼鳔
驴皮
化学成分
环磷酰胺
贫血
脏器系数
血液学指标
swim bladder
donkey skin
chemical composition
cyclophosphamide
anemia
organ coefficient
hematology index
