摘要
【目的】明确不同营养液对草地贪夜蛾Spodopterafrugiperda成虫生殖、寿命和卵巢发育的影响,为规模化饲养、精准早期监测和靶向防控提供科学依据。【方法】使用自制的营养液Ⅰ和Ⅱ为草地贪夜蛾成虫补充营养,比较不同营养液对其生殖和寿命及卵巢发育的影响。【结果】补充不同营养液对草地贪夜蛾成虫生殖和寿命均具有显著影响,其中以红糖、米酒和食醋等为主要成分的自制营养液Ⅰ效果最好,雌成虫产卵量为(1370.2±269.5)粒,卵孵化率为99.1%±0.9%、百粒卵重为(8.2±1.7)mg、雌虫寿命为(13.4±2.3)d、雄虫寿命为(12.4±2.4)d、产卵期为(6.8±1.7)d,其中产卵量和雄虫寿命与其他各处理均差异显著(P<0.05),其次为自制营养液Ⅱ,雌蛾产卵量为(1217.2±274.0)粒、卵孵化率为98.3%±1.3%、百粒卵重为(7.7±1.1)mg、雌虫寿命为(12.7±2.1)d;雄虫寿命为(10.4±1.8)d、产卵历期为(6.2±1.5)d,其中产卵量、孵化率、百粒卵重、产卵前期、雌成虫寿命和产卵历期均与对照差异显著(P<0.05)。补充营养液Ⅰ的产卵量和雄虫寿命显著高于营养液Ⅱ。卵巢解剖发现,补充不同营养液对卵巢发育等级无显著影响,羽化3 d后的卵巢发育等级均为三级,不同营养液之间差异不显著,4种营养液均显著提高了腹腔及卵巢附着的脂肪体含量。营养液Ⅰ、营养液Ⅱ、5%蔗糖水和10%蜂蜜水脂肪体重量分别为(17.9±0.4)、(16.3±1.1)、(17.1±0.5)和(17.0±0.6)mg,均显著高于灭菌蒸馏水处理(11.4±1.2)mg(P<0.05)。【结论】以红糖、米酒和食醋等为主要成分的自制营养液I对草地贪夜蛾成虫生殖力和寿命具有显著促进作用,且增加了卵巢脂肪体含量,适合在规模化饲养中为草地贪夜蛾成虫补充营养。
[Objectives]To determine the effects of different nutrient solutions on the reproduction,life span and ovarian development of adult Spodoptera frugiperda.[Methods]Four different nutrient solutions were provided to captive raised adult S.frugiperda and the effects of each of these on reproduction,life span and ovarian development were compared.[Results]Two nutrient solutions significantly improved the reproduction and longevity.Adults provided with solution I had significantly higher egg production,hatching rate,weight per hundred eggs,female longevity,male longevity and egg laying period than the control[(1370.2±269.5),(99.1%±0.9%),(8.2±1.7)mg,(13.4±2.3)d,(12.4±2.4)d and(6.8±1.7)d,respectively).The same parameters of adults that were provided with solution were(1163.7±304.7),(98.2%±2.1%),(7.4±1.0)mg,Ⅱ(11.9±2.1)d,(10.4±1.8)d,(6.1±1.7)d respectively.Of these,only egg production,hatching rate,weight per hundred eggs,preoviposition period,female longevity and oviposition period,were significantly higher than those of the control.Furthermore,adults fed on either solution I or solutionⅡalso differed significantly in the amount of eggs laid,weight per hundred eggs,female and male longevity.There was,however,no significant difference in ovarian development between females fed different nutrient solutions.All 4 nutrient solutions significantly increased the size of the fat body attached to the abdominal cavity and ovary.The fat body weights of adults fed either solution I,solution II,5%sucrose water or 10%honey water,were(17.9±0.4)mg,(16.3±1.1)mg,(17.1±0.5)mg and(17.0±0.6)mg,respectively,all of which were significantly higher than the fat body weights of adults that were provided with sterilized distilled water[(11.4±1.2)mg].[Conclusion]Nutrient solution I,the main components of which were brown sugar,rice wine and vinegar,significantly improved the fecundity and longevity of captive S.frugiperda adults,and increased the size of the ovarian fat body.This solution is a suitable nutritional supplement for captive S.frugiperda adults.
作者
张俊逸
田彩红
付俊锋
刘晓光
李国平
黄建荣
王根松
封洪强
尹新明
ZHANG Jun-Yi;TIAN Cai-Hong;FU Jun-Feng;LIU Xiao-Guang;LI Guo-Ping;HUANG Jian-Rong;WANG Gen-Song;FENG Hong-Qiang;YIN Xin-Ming(College of Plant Protection,Henan International Laboratory for Green Pest Control,Henan Agricultural University,Zhengzhou 450002,China;Biological Pesticides Engineering Research Center of Henan Province,International Joint Research Laboratory for Crop Protection of Henan,Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China,Ministry of Agriculture and Rural Affairs of the People's Republic of China,Henan Key Laboratory of Crop Pest Control,Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Yuzhou Plant Protection&Plant Quarantine Station,Yuzhou 461600,China)
出处
《应用昆虫学报》
CSCD
北大核心
2023年第4期1148-1156,共9页
Chinese Journal of Applied Entomology
基金
国家重点研发计划(2021YFD1400701)
河南省科技攻关项目(232103810016
222102240057)
河南省农业科学院科技创新团队(2023TD13)
河南省农业科学院基础性科研工作(2022JC14)
国家现代农业产业技术体系(CARS-27)
中原科技创新领军人才(224200510018)。
关键词
草地贪夜蛾
营养液
生殖力
寿命
卵巢发育
Spodoptera frugiperda
nutrient solutions
fecundity
longevity
ovarian development
