摘要
目的:有效控制石斑鱼冷藏期间的腐败变质。方法:采用Illumina高通量测序技术,通过对细菌16S rDNA V3-V4区段进行扩增,分析斜带石斑鱼经400 MPa超高压处理5 min后在4℃冷藏3,6,9,12 d的细菌菌群结构变化。结果:Alpha多样性分析表明,随着冷藏时间的延长,经超高压处理与未经超高压处理鱼样的Chao1、Ace指数不断上升,而Shannon指数却逐渐下降,但在冷藏的各个阶段(3~12 d),经超高压处理鱼样对应的Chao1、Ace和Shannon指数均低于未经超高压处理鱼样。菌群结构分析表明,两种处理鱼样在冷藏过程中的菌群结构变化不尽相同,未经超高压处理鱼样在冷藏期间出现的主要优势菌群包括Streptococcus(17.70%,3 d)、Plesiomonas(36.66%,6 d)、Peptostreptococcus(67.65%,9 d;43.4%,12 d),而经超高压处理鱼样分别为unclassified Enterobacteriaceae(27.36%,3 d)、Plesiomonas(40.31%,6 d)、Lactococcus(24.45%,9 d)和Peptostreptococcus(77.91%,12 d);确定了Peptostreptococcus为两种处理鱼样冷藏后期(第12天)的特定优势菌。PICRUSt功能基因预测发现,未经超高压处理与经超高压处理鱼样中与胁迫响应、毒物转运、DNA重组及信号转导相关的基因丰度分别于冷藏的第9天和第12天显著上升。结论:与未经超高压处理相比,经超高压处理能有效降低斜带石斑鱼在冷藏过程中的细菌菌群丰度与多样性,改变斜带石斑鱼在冷藏期间的主要优势菌群的演替规律,影响鱼样在冷藏期间组成菌群的丰度结构变化。
Objective:This study focused on the effectively control of the spoilage and deterioration of grouper during refrigeration.Methods:Illumina high-throughput sequencing technology was used to amplify the 16S rDNA V3-V4 segment of bacteria,then analyzed the changes in the bacterial community structure of E.obliquus after being treated with 400 MPa UHP for 5 minutes and refrigerated at 4℃for 3,6,9,and 12 d.Results:According to Alpha diversity analysis fish samples treated with and without UHP showed increasing Chao1 and Ace indices as refrigeration times were extended,but Shannon indices progressively dropped.Nonetheless,the fish samples treated with UHP had lower Chao1,Ace,and Shannon indices than the ones not treated with UHP at every refrigeration stage(3~12 days).Analysis of microbial community structure showed there was a difference in the changes in microbial community structure during the refrigeration process of the two types of fish samples.During the refrigeration period,the main dominant microbial communities in fish samples without UHP were Streptococcus(17.70%,3 d),Plesiomonas(36.66%,6 d),and Peptostreptococcus(67.65%,9 d;43.4%,12 d),while the main dominant microbial communities in fish samples treated with UHP were unclassified Enterobacteriaceae(27.36%,3 d),Plesiomonas(40.31%,6 d),Lactococcus(24.45%,9 d),and Peptostreptococcus(77.91%,12 d).Meanwhile,Peptostreptococcus was identified as the specific dominant bacteria for the later stage of cold storage(12th day)of two processed fish samples.PICRUSt functional gene prediction found that the relative abundance of genes related to stress response,toxin transport,DNA recombination,and signal transduction in fish samples treated with and without UHP increased significantly on the 9th and 12th days of refrigeration,respectively.Conclusion:Compared with the samples without UHP,UHP treatment can effectively reduce the abundance and diversity of bacterial communities,change the succession pattern of the main dominant bacterial communities,and affect the abundance structure changes of the bacterial communities in the fish samples during refrigeration.
作者
黄丽
庞庭才
祁岑
陈德强
HUANG Li;PANG Tingcai;QI Cen;CHEN Deqiang(Beibu Gulf University,Qinzhou,Guangxi 535011,China;Guangxi College and University Key Laboratory of High-value Utilization of Seafood and Prepared Food in Beibu Gulf,Qinzhou,Guangxi 535011,China)
出处
《食品与机械》
CSCD
北大核心
2023年第12期126-132,共7页
Food and Machinery
基金
广西科技计划项目(编号:桂科AD19245119)
北部湾大学引进高层次人才科研启动项目(编号:2019KYQD11)。
关键词
高通量测序
超高压
斜带石斑鱼
细菌多样性
high-throughput sequencing
ultra-high pressure
Epinephelus coioides
bacterial diversity
