摘要
谷子遗传资源多样性的研究对谷子基础研究与育种实践具有重要意义。选择高质量的SSR标记对DUS(Distinctness-特异性、Uniformity-一致性和Stability-稳定性)测试标准品种进行遗传多样性分析,不仅能够解析标准品种的遗传信息,也有助于对新育成品系进行分子水平的遗传多样性分析。研究利用高质量的20个SSR标记对30份不同地理来源的谷子DUS测试标准品种进行遗传多样性分析,并构建其指纹图谱;同时,利用该指纹图谱的SSR标记对22份春谷区和30份夏谷区区试品种进行遗传多样性分析,旨在为谷子品种资源利用与遗传改良奠定理论基础。结果表明,20对SSR标记在DUS测试品种平均每对引物检出的等位变异数为9.15个,20个位点的平均多态性信息含量(PIC)为0.77。利用上述SSR标记对来自春谷区与夏谷区的共计52份谷子区试品种进行分子鉴定,春谷区的聚类分析结果显示,22份春谷区区试品种分为3个主要的类群;而夏谷区的聚类分析结果显示,30份谷子夏谷区区试品种分为4个类群,但难以区分春谷、夏谷区区试品种。研究结果厘清了谷子DUS测试标准品种和区试品系间的遗传相似性。
The research on genetic resources diversity is of great significance for fundamental research and breeding practice in foxtail millet,and the screening of high quality SSR markers for the genetic diversity analysis of DUS(Distinctness,Uniformity,and Stability)test standard varieties could not only resolve the genetic information of standard varieties,but also help to analyze the genetic diversity of new lines at the molecular level.In this study,20 SSR markers with high quality were applied to analyze the genetic diversity of 30 foxtail millet varieties from different geographic origins,the varieties had been already DUS test standard varieties and used to construct the fingerprint map.Simultaneously,using the SSR markers in the fingerprint map,the genetic diversity of 22 regional test varieties in the spring foxtail millet accessions and 30 regional test varieties in the summer foxtail millet accessions were analyzed,which would lay the theoretical basis for the germplasm utility and genetic improvement of foxtail millet.The results showed that 20 SSR markers showed average 9.15 alleles per primers in the DUS test standard varieties and the average polymorphism information content(PIC)of the 20 loci was 0.77.Using the above SSR markers,molecular identification was conducted on the total of 52 millet regional test varieties in the spring foxtail millet accessions and the summer foxtail millet accessions,the cluster analysis of the varieites in the spring foxtail millet accessions indicated that 22 varieties were divided into three groups and the cluster analysis of the varieites in the summer foxtail millet accessions indicated that 30 varieties were divided into four groups,but it was difficult to distinguish the varieties in the spring and summer accessions.The genetic similarity of the DUS test standard varieties and the regional test lines of foxtail millet was elucidated by the results in this study.
作者
史慎奎
祁东梅
王春芳
王玉芳
蔡爽
SHI Shenkui;QI Dongmei;WANG Chunfang;WANG Yufang;CAI Shuang(College of Biology and Food Science,Hebei Normal University for Nationalities,Chengde 067000,China;Key Laboratory of Botany,National Ethnic Affairs Commission,Hebei Normal University for Nationalities,Chengde 067000,China;Industry Institute of Clean Energy(Carbon Emission Peak and Carbon Neutrality),Chengde 067000,China)
出处
《山西农业科学》
2024年第1期10-18,共9页
Journal of Shanxi Agricultural Sciences
基金
承德高新区第三批汇智领创空间科技创新项目(HZLC2024009)
河北民族师范学院青年基金项目(QN201601)
承德清洁能源(双碳)产业研究院项目(202205B090)。
关键词
谷子
指纹图谱
遗传多样性
DUS测试
区试品种
foxtail millet
fingerprint
genetic diversity
DUS test
regional test lines
