摘要
液体活检技术的兴起为黑色素瘤的快速、准确诊断提供了新的机遇。然而,普通循环肿瘤细胞活检基于上皮黏附蛋白进行阳性富集,但信号标记的有机荧光探针存在量子效率低的问题,导致检测黑色素瘤循环肿瘤细胞时准确率和灵敏度较低。本文以高量子效率的金属卤化物钙钛矿量子点作为信号标记物,以黑色素瘤来源的外泌体作为生物识别分子,构建了一种用于黑色素瘤液体活检的循环肿瘤细胞检测新策略。与商品化的上皮细胞黏附蛋白富集策略相比,本研究报道的复合探针检测新策略,其检测灵敏度提高了一个数量级,并且具有良好的亲水性和低毒性。实验结果证明了外泌体引导的金属卤化物钙钛矿量子点指示的黑色素瘤循环肿瘤细胞检测新策略具有理想的应用前景。
The emerging technique of liquid biopsy opens up a new opportunity for the rapid and accurate diagnosis of melanoma tumor featuring high metastatic tendency and mortality rates.However,the accuracy and sensitivity for liquid biopsy of melanoma are still limited,due to the use of low-expression epithelial cell adhesion molecule(Ep-CAM)to achieve enrichment of circulating tumor cells(CTCs)and subaltern photoluminescence quantum yields(PLQYs)organic probes as signal indicators.Here,we construct a new strategy of CTCs detection for liquid biopsy of melanoma by employing high-PLQYs metal halide perovskite(MHP)quantum dots and melanoma-derived exosome(MEX)as signal indicators and recognition analytes,respectively.The synthesized composites(PSPE)exhibit the superiorities on perfect hydrophilicity and hypotoxicity.In contrast to commercial EpCAM-triggered products,the de-tection sensitivity of our reagent materials is raised up by an order of magnitude.All results predict the potential of exosome-triggered MHP-mediated CTCs detection in accurate melanoma diagnosis of liquid biopsy.
作者
陈志山
徐朋飞
张绍安
李杨
CHEN Zhishan;XU Pengfei;ZHANG Shaoan;LI Yang(School of Biomedical Engineering,Guangzhou Medical University,Guangzhou 511436,China)
出处
《发光学报》
EI
CAS
CSCD
北大核心
2024年第1期157-168,共12页
Chinese Journal of Luminescence
基金
国家自然科学基金(52172083)
广东省国际科技合作计划(2021A0505030078)
广州市重点研发计划(2023B03J1239)
广州市教育系统创新学术团队计划(202235404)
中国博士后科学基金(2022M720919)。
