摘要
目的:考察茵陈总黄酮含量的测定方法,测定总黄酮含量。方法:以茵陈为研究药材,芦丁为对照品,建立紫外分光光度法测定茵陈总黄酮含量,检测波长511 nm,评价总黄酮百分含量,筛选显色系统,考察不同提取方法。结果:运用醇提法,显色系统为亚硝酸钠-硝酸铝-氢氧化钠,芦丁浓度在5.5~27.5μg/mL时与吸光度呈良好的线性关系。结论:茵陈提取物紫外分光光度法含量测定及总黄酮含量测定方法准确可靠、简便可行,可为茵陈黄酮类化合物富集纯化提供理论依据。
Objective:To investigate the determination method of total flavonoid content in Artemisiae Scopariae Herba,and determine the total flavonoid content.Methods:Using Artemisiae Scopariae Herba as the research herb and rutin as the reference substance,ultraviolet spectrophotometry was established to determine the content of total flavonoids in Artemisiae Scopariae Herb at a wavelength of 511 nm.The percentage content of total flavonoids was evaluated,the chromogenic system was screened,and different extraction methods were investigated.Results:Using alcohol extraction method,sodium nitrite-aluminum nitrate-sodium hydroxide was the chromogenic system,and the concentration of rutin was 5.5-27.5μg/mL.There was a good linear relationship with the absorbance.Conclusion:The ultraviolet spectrophotometry determination and total flavonoid content determination of Artemisiae Scopariae Herba extract are accurate,reliable,simple and feasible,and could provide theoretical basis for the enrichment and purification of flavonoids from Artemisiae Scopariae Herba.
作者
林文新
黄健
李承龙
Lin Wenxin;Huang Jian;Li Chenglong(Guangzhou Dermatosis Preventing&Curing Institute,Guangzhou 510095,Guangdong Province,China;Lingnan Normal University,Zhanjiang 524048,Guangdong Province,China)
出处
《中外医药研究》
2023年第35期3-5,共3页
JOURNAL OF CHINESE AND FOREIGN MEDICINE AND PHARMACY RESEARCH
关键词
茵陈
总黄酮
醇提法
Artemisiae Scopariae Herba
Total flavonoid
Alcohol extraction method
