摘要
目的:探讨膜联蛋白A6(AnnexinA6,ANXA6)基因单核苷酸多态性(single nucleotide polymorphism,SNP)及其表达水平与慢性牙周炎的相关性。方法:选择2022年1月~2022年12月于新疆医科大学第二附属医院口腔科就诊的142例慢性牙周炎患者作为研究对象,并选择同期于我院进行健康体检的150名牙周健康者作为健康对照组。对ANXA6基因的2个SNP位点rs11960458(C>T)、rs4958897(C>T)进行基因分型。采用实时荧光定量聚合酶链式反应(qRT-PCR)对ANXA6基因mRNA相对表达水平进行测定。结果:慢性牙周炎患者和健康对照组的性别、年龄、BMI、吸烟史和饮酒史比较差异均无统计学意义(P>0.05)。对于rs11960458位点,慢性牙周炎组和健康对照组CC、CT和TT基因型分布比较有统计学差异(P=0.002);两组相加模型和显性模型比较有统计学意义(P<0.05)。对于rs4958897位点,两组CC、CT和TT基因型分布和遗传模型分布均无统计学差异(P>0.05)。两组rs11960458位点C、T基因等位基因频率比较差异有统计学意义(P=0.005),而rs4958897位点C、T基因等位基因频率比较差异无统计学意义(P=0.089)。慢性牙周炎患者ANXA6基因mRNA相对表达水平显著高于健康对照组(P<0.05)。应用ANXA6基因mRNA相对表达水平区分慢性牙周炎患者和健康对照人群曲线下面积(area under the curve,AUC)为0.876(95%CI 0.835~0.916,P<0.001),灵敏度和特异度分别为72.5%和89.3%。rs11960458位点CT、TT基因型的患者ANXA6基因mRNA相对表达水平均显著高于CC基因型(P<0.05),但CT、TT基因型ANXA6基因mRNA相对表达水平比较无统计学差异(P>0.05)。结论:ANXA6基因rs11960458变异及其基因表达水平与慢性牙周炎的发生有关。
Objective:To investigate the relationship between AnnexinA6(ANXA6)gene single nucleotide polymorphism(SNP)and its expression level and chronic periodontitis.Methods:Totally 142 patients with chronic periodontitis who were treated in Department of Stomatology,Second Affiliated Hospital of Xinjiang Medical University from January 2022 to December 2022 were selected as the study objects,and 150 healthy subjects who underwent physical examination in our hospital during the same period were selected as the healthy control group.Two SNP loci rs11960458 and rs4958897 of ANXA6 gene were identified by capillary electrophoresis and fragment analysis(SNaPshot).The relative mRNA expression of ANXA6 gene was determined by qRT-PCR.Results:No significant differences were found in gender,age,BMI,smoking history and drinking history between chronic periodontitis patients and healthy control group(P>0.05).For rs11960458,there were significant differences in CC,CT and TT genotypes between the chronic periodontitis group and the healthy control group(P=0.002).The comparison of additive model and dominant model between the two groups was statistically significant(P<0.05).For rs4958897,there were no significant differences in CC,CT and TT genotype distribution and genetic model distribution between chronic periodontitis group and healthy control group(P>0.05).There was significant difference in allele frequencies of C and T genes at rs11960458(P=0.005),but no significant difference in allele frequencies of C and T genes at rs4958897(P=0.089)between the two groups.The relative mRNA expression levels of ANXA6 gene in chronic periodontitis patients were significantly higher than those in healthy controls(P<0.05).The area under the curve(AUC)to distinguish chronic periodontitis patients from healthy controls using ANXA6 gene mRNA relative expression level was 0.876(95%CI 0.835~0.916,P<0.001).The sensitivity and specificity were 72.5%and 89.3%respectively.The relative mRNA expression levels of ANXA6 gene in patients with CT and TT genotype at rs11960458 were significantly higher than those in patients with CC genotype(P<0.05),but there was no significant difference in the relative mRNA expression levels of ANXA6 gene in patients with CT and TT genotype(P>0.05).Conclusion:The variation of rs11960458 in ANXA6 gene and the level of ANXA6 gene expression are related to the onset of chronic periodontitis.
作者
赵璐
周晶
孙江伟
李亮
ZHAO Lu;ZHOU Jing;SUN Jiang-wei;LI Liang(Department of Stomatology,The Second Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830000,China)
出处
《临床口腔医学杂志》
2023年第12期730-734,共5页
Journal of Clinical Stomatology
基金
新疆维吾尔自治区自然科学基金资助项目(2022D01C786)。
关键词
慢性牙周炎
膜联蛋白A6
单核苷酸多态性
易感性
Chronic periodontitis
Annexin A6
Single nucleotide polymorphism
Susceptibility
