摘要
为了研究ASIP基因在中国美利奴细毛羊被毛颜色形成过程中的作用机制,试验分别选取体况良好的3只野生型纯白色中国美利奴细毛羊,20只纯白色、8只棕色和10只黑色ASIP基因编辑中国美利奴细毛羊背部的皮肤组织,通过H.E.染色对比样本皮肤组织结构及黑色素分布差异;采用免疫组织化学染色检测ASIP基因编辑中国美利奴细毛羊白色、棕色、黑色3种皮肤组织中ASIP蛋白的表达定位情况。结果表明:野生型与ASIP基因编辑中国美利奴细毛羊的皮肤均由表皮、真皮、皮下结缔组织3部分组成,且表皮层最薄,各毛色皮肤结构组成无差异;黑色素颗粒主要分布于黑色、棕色ASIP基因编辑中国美利奴细毛羊皮肤组织的毛囊、毛干中,且黑色较棕色皮肤组织中分布的黑色素颗粒更为密集,而在白色被毛的野生型和ASIP基因编辑中国美利奴细毛羊皮肤组织中均未发现黑色素颗粒;ASIP蛋白在白色被毛的ASIP基因编辑中国美利奴细毛羊的表皮、毛囊外根鞘、毛乳头周围的毛基质中大量存在,在棕色、黑色皮肤组织中几乎不存在。说明ASIP蛋白在特定部位的表达情况与ASIP基因编辑中国美利奴细毛羊的白色被毛毛色的发生相关。
In order to study the mechanism of ASIP in the formation of coat color in Chinese Merino fine wool sheep,the skin tissues of 3 wild type pure white,20 pure white,8 brown and 10 black ASIP gene-edited fine wool sheep with good body condition were selected.The skin tissue structure and melanin distribution differences were compared by H.E.staining technique.The expression and localization of ASIP protein in the white,brown and black skin tissues of ASIP gene-edited fine wool sheep were detected by immunohistochemical staining technique.The results showed that the skin of wild-type and ASIP gene-edited fine wool sheep was composed of epidermis,dermis and subcutaneous connective tissue,and the epidermal layer was the thinnest.Melanin particles were mainly distributed in the hair follicles and hair stem of black and brown ASIP gene edited Chinese Merino sheep skin tissue,and the melanin particles were more densely distributed in black than in brown skin tissue,while no melanin particles were found in wild type white coat and ASIP gene edited Chinese Merino sheep skin tissue.ASIP protein was abundant in the epidermis,outer root sheath of hair follicle,and the hair matrix around hair papilla of ASIP gene edited Chinese Merino sheep with white coat,but was almost absent in brown and black skin tissue.These results indicated that the expression of ASIP protein in specific parts was related to the occurrence of white coat color of Chinese Merino wool sheep edited by ASIP gene.
作者
邓雅迪
张雪梅
王旭光
DENG Yadi;ZHANG Xuemei;WANG Xuguang(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Biotechnology,Xinjiang Academy of Animal Sciences,Key Laboratory of Hereditary Breeding and Reproduction of Herbivorous Livestock,Ministry of Agriculture and Rural Areas,Key Laboratory of Animal Biotechnology,Urumqi 830026,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2024年第1期17-22,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(32160777)
新疆维吾尔自治区重点实验室开放课题项目(2019D04009)。
