摘要
目的观察化痰祛瘀汤对脑小血管病大鼠认知功能及脑组织γ-氨基丁酸(GABA)和视锥蛋白样蛋白1(VILIP-1)表达的影响,探讨其治疗脑小血管病的作用机制。方法48只雄性SD大鼠随机分为空白组、模型组、中药低剂量组和中药高剂量组,每组12只。除空白组外,采用同种系微栓子体外注入法制备脑小血管病大鼠模型,中药低、高剂量组分别予化痰祛瘀汤1.25、2.5 g/kg灌胃,空白组及模型组灌胃等量蒸馏水,连续28 d。给药1、7、14、28 d行Morris水迷宫实验评估大鼠学习记忆能力,HE染色观察海马组织病理变化,免疫组化染色检测脑组织GABA、VILIP-1蛋白表达。结果与空白组比较,模型组大鼠Morris水迷宫实验逃避潜伏期明显延长(P<0.05),穿越平台次数明显减少(P<0.05),海马组织细胞排列杂乱、间隙增宽,细胞核萎缩、坏死,脑组织GABA表达明显降低(P<0.05),VILIP-1表达明显升高(P<0.05);与模型组比较,中药低、高剂量组大鼠给药7、14、28 d逃避潜伏期明显缩短(P<0.05),穿越平台次数明显增多(P<0.05),脑组织GABA表达明显升高(P<0.05),VILIP-1表达明显降低(P<0.05);与中药低剂量组比较,中药高剂量组大鼠各时点逃避潜伏期缩短,穿越平台次数增加,海马组织病理损伤减轻,脑组织GABA表达升高,VILIP-1表达降低,差异均有统计学意义(P<0.05)。结论化痰祛瘀汤可提高海马组织GABA表达、抑制VILIP-1表达,从而改善脑小血管病大鼠认知功能。
Objective To observe the effects of Huatan Quyu Decoction on cognitive function and the expressions of GABA and VILIP-1 in brain tissue of rats with cerebral small vessel disease;To discuss its mechanism for treatment on cerebral small vessel disease.Methods Totally 48 male SD rats were randomly divided into blank group,model group,Huatan Quyu Decoction low-and high-dosage groups,with 12 rats in each group.Except for the blank group,a rat model of cerebral small vessel disease was prepared by in vitro injection of homologous microemboli.Huatan Quyu Decoction low-and high-dosage groups were given Huatan Quyu Decoction 1.25 and 2.5 g/kg by gavage,the blank group and model group were gavage with equal amounts of distilled water for 28 consecutive days.Morris water maze experiment was conducted on day 1,7,14,and 28 after administration to evaluate the learning and memory abilities of rats,HE staining was used to observe pathological changes in hippocampal tissue,and immunohistochemical staining was used to detect the expressions of GABA and VILIP-1 proteins in brain tissue.Results Compared with the blank group,the escape latency of Morris water maze experiment in model group significantly prolonged(P<0.05),and the number of crossing platforms was significantly reduced(P<0.05);the arrangement of hippocampal tissue cells was disordered,gaps widen,and nuclei atrophy and necrosis,the GABA expression in brain tissue significantly decreased(P<0.05),while the VILIP-1 expression significantly increased(P<0.05).Compared with the model group,the escape latency of Morris water maze experiment in the Huatan Quyu Decoction low-and high-dosage groups significantly shortened(P<0.05)on day 7,14,and 28 of administration,and the number of crossing platforms significantly increased(P<0.05),GABA expression significantly increased(P<0.05),while VILIP-1 expression significantly decreased(P<0.05).Compared with the Huatan Quyu Decoction low-dosage group,the escape latency of Morris water maze experiment in Huatan Quyu Decoction high-dosage group decreased at various time points,and the number of crossing platforms increase,the pathological damage of hippocampal tissue was reduced,the expression of GABA in brain tissue increased,and the expression of VILIP-1 decreased,with statistical significance(P<0.05).Conclusion Huatan Quyu Decoction can increase the expression of GABA in brain tissue and inhibit the expression of VILIP-1,thereby improve the cognitive function of rats with cerebrovascular disease.
作者
田与乾
方永军
胡亚莉
张慧
黄晓峰
韦鹏方
赵鑫雅
闫咏梅
TIAN Yuqian;FANG Yongjun;HU Yali;ZHANG Hui;HUANG Xiaofeng;WEI Pengfang;ZHAO Xinya;YAN Yongmei(Shaanxi University of Chinese Medicine,Xianyang 712000,China;Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2024年第2期123-129,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81973843)
陕西省重点研发计划(2023-YBSF-435)
中国民族医药学会科研项目(2020ZY129-410401)
陕西省创新人才推进计划-重点科技创新团队计划(2017KCT-26)
国家中医药管理局西学中骨干人才培训项目(2019年)。
