摘要
目的分析白介素-1(IL-1)、白介素-1β(IL-1β)、白介素-6(IL-6)、白介素-10(IL-10)基因多态性与糖尿病性牙周炎发生的关系。方法选取2021年6月至2022年6月石家庄市第二医院口腔科收治的糖尿病性牙周炎患者60例(观察组)及同期接受口腔检查健康人群60名(对照组)为研究对象,比较两组血清、龈沟液IL-1、IL-1β、IL-6、IL-10表达水平,检测全血DNA中IL-1、IL-1β、IL-6、IL-10基因多态性,分析其与糖尿病性牙周炎易感性的关系。结果观察组血清、龈沟液中IL-1、IL-1β、IL-6、IL-10水平明显高于对照组(t=31.987、28.911、14.201、16.562、21.315、19.146、-45.554、-57.942,P<0.05),各组龈沟液中IL-1、IL-1β、IL-6、IL-10水平明显高于血清中表达,差异有统计学意义(t=-4.080、-10.316、-10.686、10.713;t=-9.567、-6.422、-9.904、3.944,P<0.05)。观察组IL-1基因rs7413228、IL-1β基因rs2356789、IL-6基因rs5357964、IL-10基因rs4543211位点与糖尿病性牙周炎发生相关(P<0.05)。IL-1基因rs7413228位点等位基因T、IL-1β基因rs2356789位点等位基因T、IL-10基因rs4543211位点等位基因G分布频率与糖尿病性牙周炎发生相关(P<0.05)。IL-1基因rs7413228、IL-1β基因rs2356789、IL-6基因rs5357964、IL-10基因rs4543211位点多态性是糖尿病性牙周炎发生的独立影响因素(P<0.05)。结论IL-1、IL-1β、IL-6、IL-10基因多态性与糖尿病性牙周炎易感性相关,临床可通过检验患者基因多态性评估糖尿病性牙周炎发生风险。
Objective To analyze the relationship between interleukin-1(IL-1),interleukin-1β(IL-1b),interleukin-6(IL-6)and interleukin-10(IL-10)gene polymorphisms and the occurrence of diabetic periodontitis.Methods 60 patients with diabetic periodontitis admitted to the hospital from June 2021 to June 2022 and 60 healthy people who received oral examination at the same time were defined as the observation group and the control group,respectively.The expression levels of IL-1,IL-1b,IL-6 and IL-10 in serum and gingival crevicular fluid were compared between groups.IL-1,IL-1b,IL-6 and IL-10 gene polymorphisms in whole blood DNA were detected,and their relationship with the susceptibility to diabetic periodontitis was analyzed.Results The levels of IL-1,IL-1b,IL-6 and IL-10 in the serum and gingival crevicular fluid in the observation group were significantly higher than those in the control group(t=31.987,28.911,14.201,16.562,21.315,19.146,-45.554,-57.942,P<0.05).In both groups,the levels of IL-1,IL-1b,IL-6 and IL-10 in the gingival crevicular fluid were significantly higher than those in the serum(t=-4.080,-10.316,-10.686,10.713;t=-9.567,-6.422,-9.904,3.944,P<0.05).In the observation group,IL-1 rs7413228,IL-1b rs2356789,IL-6 rs5357964 and IL-10 rs4543211 were found to be associated with the occurrence of diabetic periodontitis(P<0.05).The frequencies of allele T of IL-1 rs7413228,allele T of IL-1b rs2356789 and allele G of IL-10 rs4543211 were correlated with the occurrence of diabetic periodontitis(P<0.05).The polymorphisms of IL-1 rs7413228,IL-1b rs2356789,IL-6 rs5357964 and IL-10 rs4543211 were identified as independent influencing factors of diabetic periodontitis(P<0.05).Conclusion IL-1,IL-1b,IL-6 and IL-10 gene polymorphisms are associated with susceptibility to diabetic periodontitis.In clinical practice,the risk of diabetic periodontitis can be assessed by testing for gene polymorphisms.
作者
刘佳
李芳菲
梁晓龙
牛家慧
LIU Jia;LI Fangfei;LIANG Xiaolong;NIU Jiahui(Department of Stomatology,Shijiazhuang Second Hospital,Shijiazhuang,Hebei,China,050000;Department of Stomatology,Second Outpatient Department of Institutions Directly under Hebei Province,Shijiazhuang,Hebei,China,050000)
出处
《分子诊断与治疗杂志》
2024年第1期55-58,67,共5页
Journal of Molecular Diagnostics and Therapy
基金
河北省卫生和计划生育委员会科研基金项目(20231629)。
