摘要
巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。
Nested Association Mapping(NAM)population is widely applied in genetic study and breeding practice in many crops.A NAM panel was constructed by crossing of 35 parental lines with the common maternal lines(Zhongdou 41)based on previous evaluation of soybean germplasm.Principle component analysis and clustering analysis showed that clear genetic structure was observed between subpanel of RIL populations.Genetic analysis was performed on flower color and seed coat color in NAM subpanel with significant difference between paternal and maternal parents,and we found that qFC13-1 was significantly associ-ated with flower color,which coincided with the W1 locus.Twelve loci identified were significantly correlated with seed coat color,among which nine loci were co-located by more than three methods,and the other three loci were co-located by two meth-ods,including four reported loci and eight novel loci.In conclusion,NAM population was suitable for genetic analysis of soybean,which provided material basis for genetic interpretation and breeding practice for complex traits in soybean.
作者
宋健
熊亚俊
陈伊洁
徐瑞新
刘康林
郭庆元
洪慧龙
高华伟
谷勇哲
张丽娟
郭勇
阎哲
刘章雄
关荣霞
李英慧
王晓波
郭兵福
孙如建
闫龙
王好让
姬月梅
常汝镇
王俊
邱丽娟
SONG Jian;XIONG Ya-Jun;CHEN Yi-Jie;XU Rui-Xin;LIU Kang-Lin;GUO Qing-Yuan;HONG Hui-Long;GAO Hua-Wei;GU Yong-Zhe;ZHANG Li-Juan;GUO Yong;YAN Zhe;LIU Zhang-Xiong;GUAN Rong-Xia;LI Ying-Hui;WANG Xiao-Bo;GUO Bing-Fu;SUN Ru-Jian;YAN Long;WANG Hao-Rang;JI Yue-Mei;CHANG Ru-Zhen;WANG Jun;QIU Li-Juan(Yangtze University,Jingzhou 434025,Hubei,China;National Key Facility for Gene Resources and Genetic Improvement/Beijing Key Laboratory of Soybean Biology,Ministry of Agriculture and Rural Affairs/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Agriculture,Anhui Agricultural University,Hefei 230036,Anhui,China;Crops Research Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,Jiangxi,China;Hulun Buir Institution of Agricultural Sciences,Hulun Buir 162650,Nei Mongol,China;MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Co-construction by Ministry and Province)/College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050035,Hebei,China;Jiangsu Xuhuai Regional Institute of Agricultural Sciences,Xuzhou 210031,Jiangsu,China;Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750021,Ningxia,China)
出处
《作物学报》
CAS
CSCD
北大核心
2024年第3期556-575,共20页
Acta Agronomica Sinica
基金
中国农业科学院农业科技创新计划项目(ASTIP)
云南省重点科技项目(202202AE090014)资助。
关键词
大豆
NAM群体
花色
种皮色
遗传分析
soybean
NAM population
flower color
seed coat color
genetic analysis
