摘要
大豆叶片的形状和垂直分布影响群体冠层结构和光合效率并最终影响大豆的产量。植物叶片大小、形态因着生位置不同而产生差异的现象称为异形叶,虽然异形叶现象在被子植物中广泛存在,但目前关于大豆叶片发育过程中异形叶的调控的研究还很有限。本研究通过对283份大豆种质资源的叶长、叶宽、叶形指数和异形叶指数等叶型相关的性状在江苏南京进行连续2年的考察,利用全基因组关联分析检测到181个叶型性状相关位点,其中能够在2个环境或多个性状中重复检测到的位点18个。利用检测到的与叶型相关的SNP位点,结合基因的表达谱数据、拟南芥中同源基因的功能,鉴定与大豆叶片发育和异形叶形成相关的候选基因。其中在20号染色体的相关位点Chr20:36152820上游发现已知的大豆叶形调控基因Ln(Glyma.20G116200)。此外,在19号染色体的相关位点Chr19:45155943附近鉴定到2个候选基因Glyma.19G192700、Glyma.19G194100,分别被注释为Growth-regulating factor 4(GRF4)和LITTLE ZIPPER 3(ZPR3)基因的同源基因,为阐明大豆异形叶等叶型性状遗传的分子机制奠定了基础。
Leaf shape and vertical distribution of soybean affect canopy structure,photosynthetic efficiency,and yield.The exis-tence of different leaf shapes and sizes on the same plant,which is known as heterophylly,has been observed in many flowering plant species.Yet,the genetic characteristics and genetic basis of heterophylly in soybean remain unknown.In this study,leaf characteristics such as leaf length,leaf width,leaf shape index,and heterophylly index were investigated in 283 soybean germ-plasm resources for two consecutive years in Nanjing,Jiangsu Province.A total of 181 related loci were detected by genome-wide association study(GWAS),among which 18 loci could be repeatedly detected in two environments or among multiple traits.Us-ing the loci associated with leaf characteristics,we integrated the GWAS approach with the expression profiling data and gene-based association and functional annotation of orthologs in Arabidopsis to identify candidate genes involved in leaf devel-opment in soybean.The known soybean leaf shape regulatory gene Ln(Glyma.20G116200)was found upstream of locus Chr20:36152820.In addition,two candidate genes(Glyma.19G192700 and Glyma.19G194100)were identified near the related locus Chr19:45155943 on chromosome 19,homologous genes of growth-regulating factor 4(GRF4),and LITTLE ZIPPER 3(ZPR3),respectively.These results lay a solid foundation for expanding our understanding of the genetic mechanism of hetero-phylly in soybean.
作者
王琼
朱宇翔
周密密
张威
张红梅
陈新
陈华涛
崔晓艳
WANG Qiong;ZHU Yu-Xiang;ZHOU Mi-Mi;ZHANG Wei;ZHANG Hong-Mei;CEHN Xin;CEHN Hua-Tao;CUI Xiao-Yan(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,Jiangsu,China;College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,Jiangsu,China)
出处
《作物学报》
CAS
CSCD
北大核心
2024年第3期623-632,共10页
Acta Agronomica Sinica
基金
江苏省基础研究计划(自然科学基金)项目(BK20220740)
江苏省种业振兴揭榜挂帅项目(JBGS[2021]057)
江苏省重点研发计划项目(BE2022328)
江苏省农业科技自主创新项目(CX(22)5002)资助。
