摘要
建立电喷雾检测器高效液相色谱法测定复方北豆根氨酚那敏片中灰毡毛忍冬皂苷乙和川续断皂苷乙。选用Waters XBridge C_(18)色谱柱(250 mm×4.6 mm,5μm)为分析柱,以甲醇为流动相A,0.05%三氟乙酸溶液为流动相B,梯度洗脱,检测器温度为35℃。灰毡毛忍冬皂苷乙和川续断皂苷乙的质量浓度分别在2.33~291.57μg/mL、2.42~302.40μg/mL范围内与色谱峰面积线性关系良好,相关系数分别为0.9996、0.9992,检出限分别为1.17、1.21μg/mL,定量限分别为2.33、2.42μg/mL。测定结果的相对标准偏差分别为2.54%和0.66%(n=6),样品加标平均回收率分别为104.8%和101.5%。该方法简单快捷,可为控制制剂中金银花投料的规范性提供技术支持。
A method for the determination of macranthoidin B and dipsacoside B in compound asiatic moonseed,paracetamol and chlorphenamine maleate tablets by high performance liquid chromatography-charged aerosol detector(HPLC-CAD)was established.The determination was performed on Waters XBridge C_(18)column(250 mm×4.6 mm,5μm)with methanol as the mobile phase A and 0.05%TFA(trifluoroacetic acid)as the mobile phase B in gradient elution.The temperature of CAD was 35℃.The mass concentration of macranthoidin B and dipsacoside B had a good linear relationship with the chromatographic peak area in the range of 2.33-291.57μg/mL,2.42-302.40μg/mL,respectively,and the corelation coefficients were 0.9996 and 0.9992.The detection limits were 1.17,1.21μg/mL,and the limits of quantification were 2.33,2.42μg/mL,respectively.The relative standard deviations of the determination results were 2.54%and 0.66%(n=6),and the average recoveries were 104.8%and 101.5%,respectively.The method is simple,which could provide technical support for the control of standardization adding of lonicerae japonicea flos in the preparation.
作者
沈心
顾晓风
周震宇
SHEN Xin;GU Xiaofeng;ZHOU Zhenyu(Suzhou Institute for Drug Control,Suzhou 215104,China)
出处
《化学分析计量》
CAS
2024年第1期65-69,共5页
Chemical Analysis And Meterage
关键词
复方北豆根氨酚那敏片
高效液相色谱法
电喷雾检测器
灰毡毛忍冬皂苷乙
川续断皂苷乙
金银花
compound asiatic moonseed,paracetamol and chlorphenamine maleate tablets
high performance liquid chromatography
charged aerosol detector
macranthoidin B
dipsacoside B
lonicerae japonicea flos
