摘要
【目的】旨在明确溴虫氟苯双酰胺多代汰选后对草地贪夜蛾Spodoptera frugiperda种群适合度和解毒酶活性的影响,为科学合理使用该杀虫剂提供理论基础。【方法】采用饲料表面涂毒法用0.25 mg/L溴虫氟苯双酰胺对草地贪夜蛾初孵幼虫连续汰选10代后,测定溴虫氟苯双酰胺对草地贪夜蛾3龄幼虫的毒力、3龄幼虫体内解毒酶(细胞色素P450单加氧酶、谷胱甘肽S-转移酶和羧酸酯酶)活性以及种群适合度变化,同时在对草地贪夜蛾3龄幼虫的生测中测定了3种增效剂(PBO,TPP和DEM)对溴虫氟苯双酰胺的增效作用。【结果】与未汰选种群UNSEL相比,经溴虫氟苯双酰胺汰选10代后的草地贪夜蛾汰选种群Bro-SEL的抗性倍数为2.71倍。与UNSEL种群相比,汰选种群Bro-SEL的繁殖力和幼虫存活率显著降低,内禀增长率、周限增长率和净增殖率也显著降低,相对适合度为0.35。Bro-SEL种群3龄幼虫体内羧酸酯酶活性显著升高,以α-NA和β-NA为底物时的活性分别为UNSEL种群的1.62和1.61倍,但细胞色素P450单加氧酶和谷胱甘肽S-转移酶活性无显著变化。对草地贪夜蛾3龄幼虫的生测结果显示PBO和TPP对溴虫氟苯双酰胺均具有显著增效作用,增效比分别为1.31和1.42。【结论】这些结果表明汰选10代后溴虫氟苯双酰胺对草地贪夜蛾种群的毒力变化很小,说明草地贪夜蛾对溴虫氟苯双酰胺的代谢抗性风险较低。溴虫氟苯双酰胺这一杀虫剂连续汰选对其种群发育和繁殖存在较高的适合度代价。溴虫氟苯双酰胺是一种防治草地贪夜蛾良好的替代药剂。
【Aim】To clarify the effects of broflanilide on the fitness and detoxification enzyme activities of Spodoptera frugiperda after multiple generations of selection and to provide a theoretical basis for the scientific and rational use of this insecticide.【Methods】The changes in the toxicity of broflaniliole to the 3rd instar larvae,the activities of detoxification enzyms(cytochrome P450 monooxygenase,glutathione S-transferase and carboxylesterase)in the 3rd instar larvae and the population fitness of S.frugiperda were determined after the newly hatched larvae were continuously exposed to 0.25 mg/L of broflanilide for 10 generations by the diet-overlay method.And the synergistic effects of three synergists(PBO,TPP and DEM)on broflanilide were determined in bioassay against the 3rd instar larvae of S.frugiperda.【Results】After 10 generations of selection by broflanilide,the exposed S.frugiperda population Bro-SEL developed 2.71-fold resistance to broflanilide,compared to the unexposed population UNSEL.At the same time,the fecundity and larval survival rate of the exposed population Bro-SEL were significantly decreased and the population parameters(intrinsic rate of increase,finite rate of increase and net reproductive rate)were also significantly decreased,as compared to those in UNSEL.The Bro-SEL population had the relative fitness of 0.35.The carboxylesterase activities in the 3rd instar larvae of Bro-SEL were significantly increased by 1.62-and 1.61-fold,respectively,when usingα-NA andβ-NA as the substrates,but the activities of cytochrome P450 monooxygenase and glutathione S-transferase in the 3rd instar larvae of Bro-SEL had no significant change as compared with those in the unexposed population UNSEL.Bioassay against the 3rd instar larvae of S.frugiperda revealed that both PBO and TPP had significant synergistic effects on broflanilide,with the synergistic ratios of 1.31 and 1.42,respectively.【Conclusion】These results showed that there was little change in the toxicity of broflanilide to S.frugiperda population after 10 generations of selection by the insecticide broflanilide,indicating that S.frugiperda has a low risk of metabolic resistance to broflanilide,but the population development and reproduction of S.frugiperda have high fitness cost,and broflanilide is a good alternative agent for controlling S.frugiperda.
作者
姬雪娇
崔丽
王文杰
杨庆杰
翟艳慧
芮昌辉
JI Xue-Jiao;CUI Li;WANG Wen-Jie;YANG Qing-Jie;ZHAI Yan-Hui;RUI Chang-Hui(Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2023年第12期1570-1580,共11页
Acta Entomologica Sinica
基金
国家重点研发计划项目(2022YFD1400904)。
关键词
草地贪夜蛾
溴虫氟苯双酰胺
适合度
解毒酶
抗药性
Spodoptera frugiperda
broflanilide
fitness
detoxification enzyme
pesticide resistance
