摘要
目的探究化橘红提取物在糖尿病肾病(diabetic nephropathy,DN)中的多靶点防治作用。方法对大鼠腹腔注射STZ试剂制备DN模型。将90只Wistar实验大鼠分为为正常对照组、模型空白组、贝那普利对照组、化橘红提取物预防组、化橘红提取物低剂量组和化橘红提取物高剂量组。检测各组FBG、HbA1C、U-mA1b、β_(2)-MG、SCr、BUN水平,同时检测SGLT2、AGES、RAGES、TGF-β1的mRNA及蛋白表达水平。结果模型空白组FBG、HbA1C、U-mA1b、β_(2)-MG、BUN水平均较正常对照组升高,SGLT2、AGES、RAGES、TGF-β1的mRNA和蛋白表达水平均较正常对照组升高,中西药物干预后上述指标均较模型空白组降低(P<0.05);其中,中药预防组干预效果最佳。结论化橘红提取物的作用机制可能是通过调节葡萄糖转运蛋白的异常表达、减少糖基化作用而减少TGF-β1表达水平,发挥防治DN、保护肾脏损伤的作用。
Objective To explore the multi-target prevention and treatment effect of Citrus grandis extract in diabetic nephropathy.Methods The rat model of diabetic nephropathy was induced by intraperitoneal injection of STZ reagent.90 Wistar experimental rats were divided into 6 groups:normal control group,model blank group,benazepril control group,orange blossom extract prevention group,orange blossom extract low-dose group,and orange blossom extract high-dose group.The levels of FBG,HbA1C,U-mA1b,β_(2)-MG,SCr,and BUN were detected in each group,and the mRNA and protein expression levels of SGLT2,AGES,RAGES,and TGF-β1 were also detected.Results The levels of FBG,HbA1C,U-mA1b,β_(2)-MG and BUN in the model blank group were higher than those in the normal control group,and the levels of mRNA and protein expression of SGLT2,AGES,RAGES,and TGF-β1 were higher than those in the normal control group,and all of the above indexes were lower than those in the model blank group after the intervention of Chinese and Western medicines(P<0.05);among them,the intervention effect of the traditional Chinese medicine prevention group was The intervention effect of the Chinese medicine prevention group was the best.Conclusions The mechanism of action of citrus extract may be to reduce TGF by regulating abnormal expression of glucose transporters and reducing glycosylation-β1 expression level,play a role in preventing and treating diabetic nephropathy and protecting kidney damage.
作者
杨帆
王岑婷
王海霞
张晨
YANG Fan;WANG Centing;WANG Haixia;ZHANG Chen(Department of Hepatobiliary Surgery,Guangzhou Medical University Affiliated Eighth Hospital,Guangzhou 510440,China;School of Clinical Pharmacy and School of Clinical Pharmacy(School of Comprehensive Pharmacy),Guangdong Pharmaceutical University,Guangzhou 528405,China;Department of Pharmacy,Third People's Hospital of Bijie,Bijie 551799,China;Department of Clinical Pharmacy,Guangzhou First People's Hospital,Guangzhou 510180,China)
出处
《中国医药指南》
2024年第4期54-58,共5页
Guide of China Medicine
基金
2021年广州市中医药和中西医结合医药科技项目(20212A010002)。
