摘要
为筛选出具有抗蜜蜂病毒作用效果的天然产物,给感染以色列急性麻痹病毒(IAPV)的蜜蜂分别饲喂含有9种天然产物(百里酚、青蒿素、梓醇、胡椒碱、可可碱、苦杏仁苷、咖啡酸、橙皮苷和ρ-香豆酸)各两种浓度的蔗糖溶液,采用RT-PCR法检测9种天然产物饲喂7 d后对蜜蜂体内病毒的抑制效果,同时测定可能有效的天然产物对蜜蜂免疫相关基因表达的影响。结果表明:外源接种IAPV病毒实验条件下,10μmol·L^(-1)百里酚饲喂7 d后IAPV病毒载量比对照组显著降低;未外源接种病毒的条件下,10μmol·L^(-1)百里酚饲喂7 d后蜜蜂携带的IAPV和蜜蜂囊状幼虫病毒(SBV)病毒载量比对照组显著降低,卵黄原蛋白基因在转录水平显著提高。经过筛选发现,蜜蜂饲喂10μmol·L^(-1)百里酚7 d后能达到较好的抗病毒效果,百里酚可成为抗蜜蜂病毒药物研发的潜力成分。
In order to identify natural products with anti-honeybee virus effects,9 natural products(thymol,artemisinin,catalpol,piperine,theobromine,amygdalin,caffeic acid,hesperidin and ρ-coumaric acid) were tested in lab conditions.RT-PCR was used to measure the inhibitory effect of the 9 natural products on honeybee viruses after 7 days.Additionally,the effects of the natural products on the expression of honeybee immunity-related genes were also determined.The results of the exogenous inoculation of Israeli acute paralysis virus(IAPV) virus suggest that feeding honeybees with 10 μmol·L~(-1) thymol for 7 days can significantly reduce the viral load of IAPV and Sacbrood virus(SBV).Without external vaccination of the virus,after feeding with 10 μmol · L~(-1) thymol for 7 days,the viral load of IAPV and SBV carried by honeybees was significantly reduced compared with the control group,and the transcription level of vitellogenin gene was significantly increased.After screening,it was found that honeybees fed with 10 μmol · L~(-1) thymol can achieve good antiviral effects after 7 days,and thymol can become a potential component in the development of anti-honeybee virus drugs.
作者
黄知楚
周倪红
刘瑶
郑火青
HUANG Zhichu;ZHOU Nihong;LIU Yao;ZHENG Huoqing(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)
出处
《浙江农业学报》
CSCD
北大核心
2024年第1期67-74,共8页
Acta Agriculturae Zhejiangensis
基金
国家蜂产业技术体系专项(CARS-44)
浙江省重大科技专项(2021C02068-8)。
关键词
蜜蜂
天然产物
病毒
免疫
honeybee
natural product
viral disease
immunity
