摘要
目的:探究牛蒡子总木脂素对2型糖尿病大鼠视网膜组织的保护作用。方法:Wistar大鼠随机分为正常组、模型组、溶媒组、双丹明目胶囊(618 mg·kg^(-1))组和牛蒡子总木脂素低、中、高剂量(100、200、400 mg·kg^(-1))组,每组16只。对相应各组大鼠采用链脲佐菌素(STZ)联合高脂饲料诱导造模后,各给药组大鼠灌胃相应药物,每日1次,连续14周;第14周,麻醉后腹主动脉取血,分离血清,摘取大鼠双侧眼球冷冻保存。采用苏木素-伊红(HE)染色观察大鼠视网膜组织组织病理变化;采用视网膜血管组织消化铺片观察大鼠视网膜血管网的病理变化;采用酶联免疫吸附测定法(ELISA)试剂盒检测血清中血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)、细胞间黏附因子-1(ICAM-1)等炎症因子的变化。结果:与正常组比较,模型组大鼠视网膜组织出现显著的病理性改变,视网膜神经节细胞数显著减少(P<0.01),视网膜厚度显著变薄(P<0.01);视网膜血管中内皮细胞与周细胞的比值(E/P)显著降低(P<0.01);血清中VEGF、TNF-α、ICAM-1水平显著升高(P<0.01)。与模型组比较,牛蒡子总木脂素视网膜神经节细胞数量显著升高(P<0.01),视网膜厚度显著增加(P<0.01);血清中VEGF、TNF-α、ICAM-1等炎症因子水平明显降低(P<0.05,P<0.01)。结论:牛蒡子总木脂素可能通过下调VEGF、TNF-α、ICAM-1水平从而发挥视网膜保护作用。
Objective:To investigate the protective effect of total lignans of Arctii Fructus on the retinal tissue in the rat model of type 2 diabetes mellitus.Method:Wistar rats were randomized into normal,model,solvent,Shuangdan Mingmu Capsules(618 mg·kg^(-1)),and low-,medium-,and high-dose(100,200,400 mg·kg^(-1),respectively)total lignans of Arctii Fructus groups,with 16 rats in each group.The rat model was established by streptozotocin(STZ)combined with a high-fat diet and administrated with corresponding drugs by gavage once a day for 14 weeks.At the 14th week,blood was sampled for the collection of serum from the abdominal aorta after anesthesia,and bilateral eyeballs were collected and frozen.Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of the retinal tissue in rats.The pathological changes of retinal vascular network in rats were observed by retinal vascular tissue digestion and mounting The levels of vascular endothelial growth factor(VEGF),tumor necrosis factor-α(TNF-α),and intercellular adhesion molecule-1(ICAM-1)in the serum were determined by the ELISA kit.Result:Compared with the normal group,the solvent group showed pathological changes in the retinal tissue,reduced retinal ganglion cells(P<0.01),and retinal thinning(P<0.01),decreased E/P value in retinal blood vessels(P<0.01),and elevated serum levels of VEGF,TNF-α,and ICAM-1(P<0.01).Compared with the model group,the total lignans of Arctii Fructus increased the retinal ganglion cells(P<0.01),thickened the retina(P<0.01),and lowered the serum levels of VEGF,TNF-α,and ICAM-1(P<0.05,P<0.01).Conclusion:Total lignans of Arctii Fructus may lower the VEGF,TNF-α,and ICAM-1 levels to protect the retina.
作者
金童
沈越
徐朝晖
王海颖
JIN Tong;SHEN Yue;XU Zhaohui;WANG Haiying(Shanghai University of Traditional Chinese Medicine(TCM),Shanghai 200120,China;Innovation Research Institute of TCM,Shanghai University of TCM,Shanghai 200120,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2024年第1期140-148,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年基金项目(81403345)
上海市科学技术委员会科研计划项目(18401900300)
上海市进一步加快中医药传承创新发展三年行动计划(2021年-2023年)项目[ZY(2021-2023)-0403]。
