摘要
目的探讨触珠蛋白基因多态性与老年血管性痴呆患者病情严重程度及疾病易感性关系。方法选取2018年2月至2023年2月该院收治的80例老年血管性痴呆患者作为血管性痴呆组,另选同期该院收治的80例非血管性痴呆脑卒中人群作为对照组。采用聚合酶链反应-序列特异性引物法测定两组触珠蛋白基因位点基因型及等位基因分布频率,并分析其与血管性痴呆患者病情严重程度及疾病易感性关系。结果血管性痴呆组高脂血症史、糖尿病史占比和总胆固醇、甘油三酯水平高于对照组,差异有统计学意义(P<0.05);各基因型分布符合Hardy-Weinberg平衡定律(P>0.05),血管性痴呆组触珠蛋白2-2基因型频率、触珠蛋白2等位基因频率高于对照组,差异有统计学意义(P<0.05);不同触珠蛋白基因型血管性痴呆患者简易精神状态量表评分、缺血指数量表评分比较差异有统计学意义(P<0.05);多因素Logistic回归分析显示,携带触珠蛋白2-2基因型、携带触珠蛋白2等位基因是影响血管性痴呆发生的独立危险因素(P<0.05)。结论触珠蛋白2-2基因型、触珠蛋白2等位基因分布频率与卒中后血管性痴呆发生有关,且触珠蛋白2-2基因型、触珠蛋白2等位基因分布频率较高者病情更为严重,可为血管性痴呆的早期识别及病情评估提供参考。
Objective To explore the relationship between haptoglobin gene polymorphism and disease severity and susceptibility in senile vascular dementia patients.Methods A total of 80 patients with senile vascular dementia admitted to the hospital from February 2018 to February 2023 were selected as the vascular dementia group,and 80 stroke patients with non-vascular dementia admitted to the hospital during the same period were selected as the control group.The genotype distribution and allele frequency of haptoglobin gene were measured using polymerase chain reaction with sequence-specific primers,and their relationship with the severity and susceptibility of vascular dementia patients was analyzed.Results The proportion of history of hyperlipidemia and diabetes mellitus and the levels of total cholesterol and triglyceride in vascular dementia group were higher than those in control group,the differences were statistically significant(P<0.05).The distribution of genotypes was in Hardy-Weinberg equilibrium(P>0.05).The frequency of haptoglobin 2-2 genotype and haptoglobin 2 allele in vascular dementia group were higher than those in control group,and the differences were statistically significant(P<0.05).There were significant differences in the scores of mini-mental state examination and hachinski ischaemic score among patients with vascular dementia with different haptoglobin genotypes(P<0.05).Multivariate Logistic regression analysis showed that the carrier of haptoglobin 2-2 genotype and the carrier of haptoglobin 2 allele were independent risk factors for vascular dementia(P<0.05).Conclusion Haptoglobin 2-2 genotype and haptoglobin 2 allele distribution frequency are associated with the occurrence of vascular dementia after stroke,and those with high frequency of haptoglobin 2-2 genotype and haptoglobin 2 allele distribution suffer a severe disease,which can provide reference for early identification and assessment of vascular dementia.
作者
陈晶
史文俊
安黎云
郑运周
徐冬梅
王缚鲲
CHEN Jing;SHI Wenjun;AN Liyun;ZHENG Yunzhou;XU Dongmei;WANG Fukun(Department of Clinical Laboratory,the 980th Hospital of the Joint Logistics Support Force of the Chinese People′s Liberation Army,Shijiazhuang,Hebei 050082,China)
出处
《国际检验医学杂志》
CAS
2024年第3期325-329,共5页
International Journal of Laboratory Medicine
基金
河北省卫建委医学科学研究课题(20231314)。
关键词
触珠蛋白
基因多态性
血管性痴呆
haptoglobin
genetic polymorphism
vascular dementia
