摘要
目的探讨不同阶段胃黏膜病变患者胃黏膜组织Wnt/β-连环蛋白(Wnt/β-catenin)信号通路、肿瘤坏死因子α诱导蛋白8样因子3(TIPE3)的表达情况及临床意义。方法选取2021年1月—2023年1月在该院确诊的胃黏膜病变患者100例作为研究对象,根据患者胃黏膜病变的不同阶段,分为慢性非萎缩性胃炎(NAG)组(n=33)、肠化生(IM)组(n=40)、胃癌(GC)组(n=27),另取同期体检健康人34例作对照组。观察受试者Hp感染情况。比较不同阶段病变胃黏膜患者炎症因子[肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-17(IL-17)、γ-干扰素(IFN-γ)]、Wnt/β-catenin信号通路相关蛋白(Wnt3α、β-catenin、GSK-3β、Wnt1)及TIPE3表达水平。结果胃黏膜病变患者Hp感染率为71.00%(71/100),高于对照组的32.35%(11/34)(P<0.05)。胃黏膜病变患者TNF-α、IL-6、IL-17、IFN-γ水平显著高于对照组,且IM组和GC组患者TNF-α、IL-6、IL-17、IFN-γ水平显著高于NAG组(P<0.05);胃黏膜病变患者Wnt3α、β-catenin、GSK-3β、Wnt1、TIPE3水平显著高于对照组,且IM组和GC组患者Wnt3α、β-catenin、GSK-3β、Wnt1、TIPE3水平显著高于NAG组(P<0.05);Wnt/β-catenin信号通路相关蛋白Wnt3α、β-catenin、GSK-3β、Wnt1分别与TNF-α、IL-6、IL-17、IFN-γ及TIPE3均呈正相关(P<0.05)。结论胃黏膜病变发展可能与Wnt/β-catenin信号通路激活,TIPE3表达水平上调有关。
Objective To explore the expressions and clinical significance of Wnt/β-catenin signaling pathways and tumor necrosis factorα-inducible protein 8-like factor 3(TIPE3)in gastric mucosal tissues in patients with gastric mucosal lesions at different stages.Methods A total of one hundred patients with gastric mucosal lesions confirmed in the hospital were enrolled as the research objects between January 2021 and January 2023.According to gastric mucosal lesions at different stages,they were divided into chronic non-atrophic gastritis(NAG)group(n=33),intestinal metaplasia(IM)group(n=40)and gastric cancer(GC)group(n=27).And other 34 healthy controls during the same period were enrolled as control group.Hp infection was observed in all patients.The levels of inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-17(IL-17),interferon-γ(IFN-γ)],Wnt/β-catenin signaling pathway related proteins[Wnt3α,β-catenin,GSK-3β,Wnt1]and TIPE3 in patients with gastric mucosal lesions at different stages were compared.Results The Hp infection rate of patients with gastric mucosal lesions was 71.00%(71/100),which was higher than that of the control group[32.35%(11/34)](P<0.05).The levels of TNF-α,IL-6,IL-17 and IFN-γin patients with gastric mucosal lesions were significantly higher than those in control group,which were significantly higher in IM and GC group than NAG group(P<0.05).The levels of Wnt3α,β-catenin,GSK-3β,Wnt1 and TIPE3 in patients with gastric mucosal lesions were significantly higher than those in control group,which were significantly higher in IM and GC group than NAG group(P<0.05).Wnt/β-catenin signaling pathway related proteins Wnt3α,β-catenin,GSK-3β and Wnt1 were positively correlated with TNF-α,IL-6,IL-17,IFN-γand TIPE3(P<0.05).The levels of Wnt3α,β-catenin,GSK-3β,Wnt1,TIPE3,TNF-α,IL-6,IL-17 and IFN-γin Hp-positive group were significantly higher than those in Hp-negative group(P<0.05).Conclusion The development of gastric mucosal lesions may be related to the activation of Wnt/β-catenin signaling pathways and the up-regulation of TIPE3 expression.
作者
严展
YAN Zhan(Meishan Hospital of Traditional Chinese Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 620500,China)
出处
《中国煤炭工业医学杂志》
2023年第6期625-629,共5页
Chinese Journal of Coal Industry Medicine
基金
四川省医学科研课题立项计划项目(编号:S17083)。
