摘要
目的探讨气滞胃痛颗粒对大鼠急性胃炎的治疗作用,初步揭示其作用机制。方法将56只雄性SPF级Wistar大鼠随机分为空白组、模型组、阳性组和气滞胃痛颗粒低、中、高剂量组,每组各8只。采用70%乙醇诱导大鼠急性胃炎模型,给药治疗后HE染色观察各组大鼠胃组织病理变化,ELISA法检测血清TNF-α、SOD、TGF-β含量,Western blot法检测胃组织INOS、HIF-1α、IFNG蛋白表达水平。结果气滞胃痛颗粒组大鼠治疗后,急性胃炎大鼠胃出血斑点较模型组有了较大改善,胃部褶皱逐渐恢复,色泽更为光亮。胃组织结构基本完整,主细胞数量略有缺失,组织间隙分布有少量血细胞,无炎性细胞浸润,整体结构比较完整。大鼠血清中TNF-α和TGF-β的含量均明显下降(P<0.05),SOD的含量则明显上升(P<0.05)。胃组织INOS、HIF-1α、IFNG(IFN-γ)蛋白表达均明显降低(P<0.05)。结论气滞胃痛颗粒对大鼠急性胃炎具有较好的治疗作用,其作用机制与HIF-1信号通路有关。
Objective To explore the therapeutic effect of Qizhiweitong Granule on acute gastritis in rats and preliminarily reveal its mechanism of action.Methods A total of 56 male SPF Wistar rats were randomly divided into blank group,model group,positive group and low-,medium-and high-dose groups of Qizhiweitong Granule,with 8 rats in each group.Acute gastritis in rats was induced by 70%ethanol,and after drug treatment,the pathological changes in rat gastric tissue were observed using HE staining.The contents of TNF-α,SOD and TGF-βin serum were detected by ELISA,and the expression levels of INOS,HIF-1αand IFNG in gastric tissue were determiend by Western blot.Results After the treatment with Qizhiweitong Granule,the gastric bleeding spots in rats with acute gastritis were significantly improved compared with the model group,with gradual recovery of gastric folds and a brighter color.The structure of gastric tissue was basically intact,with a slight loss of chief cells,a few blood cells in the interstitial space,no inflammatory cell infiltration,and overall structural integrity.The contents of TNF-αand TGF-βin serum of rats were significantly decreased(P<0.05),while the content of SOD was significantly increased(P<0.05).The expressions of INOS,HIF-1αand IFNG(IFN-γ)in gastric tissues were significantly decreased(P<0.05).Conclusion Qizhiweitong Granule have an excellent therapeutic effect on acute gastritis in rats,and its mechanism of action is related to the HIF-1 signaling pathway.
作者
王帅
王亮
秦鑫鹏
韩凌
包永睿
李天娇
王萍
杨洋
张涛
魏玮
孟宪生
WANG Shuai;WANG Liang;QIN Xin-peng;HAN Ling;BAO Yong-rui;LI Tian-jiao;WANG Ping;YANG Yang;ZHANG Tao;WEI Wei;MENG Xian-sheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian Liaoning 116600;Liaoning Provincial TCM Multi-Dimensional Analysis Technical Innovation Center,Dalian Liaoning 116600;Liaoning Engineering Laboratory of Modern Chinese Medicine Research,Dalian Liaoning 116600;China Resources Sanjiu Pharmaceutical Co.,Ltd.,Shenzhen Guangdong 518110;Department of Spleen and Stomach Diseases,Wangjing Hospital of China Academy of Chinese Medical Sciences,Beijing 100102)
出处
《世界中西医结合杂志》
2024年第1期84-89,共6页
World Journal of Integrated Traditional and Western Medicine
基金
辽宁省博士科研启动基金项目(2023-BS-138)。
