摘要
目的:利用聚多巴胺仿生法在聚己内酯(PCL)螺钉表面制备羟基磷灰石(HA)涂层,评价其对前交叉韧带重建后骨整合的影响。方法:通过3D打印设计制作多孔PCL螺钉,利用聚多巴胺仿生法制备HA涂层。选取20只成年雄性新西兰大白兔,于双侧后肢建立前交叉韧带重建模型,左右侧随机植入含HA涂层(实验组)及不含HA涂层的(对照组)PCL螺钉,术后6、12周分别处死10只,取膝关节,制备股骨-前交叉韧带-胫骨复合物标本,通过Masson染色、Micro-CT检测、生物力学测试评价螺钉与骨组织界面的骨整合情况。结果:Masson染色结果示术后6、12周两组螺钉周围均有较多新生骨小梁形成并向螺钉孔隙内生长,但实验组新生骨小梁的量和深度均优于对照组。Micro-CT示术后6、12周两组螺钉界面骨隧道内新生骨逐渐增加,且实验组周围新生骨量多于对照组。与术后6周相比,两组术后12周骨体积分数、骨小梁数量、骨密度均升高,骨小梁分离率降低;术后6、12周,实验组以上指标均优于对照组(P<0.05)。术后6、12周实验组最大拉力峰值均高于对照组[(39.98±5.32)N vs(27.46±6.95)N,(62.64±8.76)N vs(50.15±6.48)N](P<0.05)。结论:聚多巴胺仿生法制备的PCL螺钉的HA涂层能够促进前交叉韧带重建术后骨整合,提高界面螺钉生物力学稳定性。
Aim:To prepare hydroxyapatite(HA)coating on polycaprolactone(PCL)screws by polydopamine biomimetic method,and evaluate the effects on osseointegration in anterior cruciate ligament reconstruction.Methods:The porous PCL screw was designed and printed by 3D printer.HA coating was prepared by polydopamine biomimetic method.Twenty adult male New Zealand white rabbits were selected to establish the model of anterior cruciate ligament reconstruction in the bilateral hindlimbs and randomly allocated into 2 groups,and PCL screws with HA coating(experimental group)and without HA coating(control group)were implanted.Ten rabbits were killed 6 and 12 weeks after operation,and the specimens of femur-anterior cruciate ligament-tibia complex were prepared.The osseointegration of screw bone interface was evaluated by Masson staining,Micro-CT and biomechanical test.Results:Masson staining showed that at 6 and 12 weeks after operation,more new bone trabeculae formed around the material and grew into the pores of the material,but the amount and depth of new bone trabeculae in the experimental group were better than those in control group.Micro-CT showed that the new bone in the material interface of the 2 groups gradually increased at 6 and 12 weeks after operation,and the new bone mass around the experimental group was more than that of control group.Compared with 6 weeks after operation,the bone volume fraction,trabecular number and bone mineral density increased,while the trabecular separation rate decreased in both groups at 12 weeks after operation,and the above indexes in the experimental group were better than those in control group at 6 and 12 weeks after operation(P<0.05).At 6 and 12 weeks after operation,the peak tension of the experimental group was higher than that of control group[(39.98±5.32)N vs(27.46±6.95)N,(62.64±8.76)N vs(50.15±6.48)N](P<0.05).Conclusion:The HA coating of PCL screws prepared by polydopamine biomimetic method can promote osseointegration after anterior cruciate ligament reconstruction and improve the biomechanical stability of interface screws.
作者
任铖真
毕方刚
王志远
王亚飞
李鹏举
田科
REN Chengzhen;BI Fanggang;WANG Zhiyuan;WANG Yafei;LI Pengju;TIAN Ke(The Sixth Ward,Department of Orthopedics,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Knee Joint Ward,Department of Joint Surgery,Xi'an Honghui Hospital,Xi′an 710000)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2024年第1期40-44,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关计划项目(202102310214)
陕西省重点研发计划(S2017-ZDYF-YBXM-SF-0764)
河南省高等学校重点科研项目(22A320014)。
关键词
前交叉韧带重建术
羟基磷灰石涂层
聚多巴胺仿生法
骨整合
兔
anterior cruciate ligament reconstruction
hydroxyapatite coating
polydopamine biomimetic method
osseointegration
rabbit
