摘要
目的优化红三七炮制工艺,并评价其补血活性。方法在单因素试验基础上,以蒸制温度、蒸制时间、烘干温度、烘干时间为影响因素,三七皂苷R1,人参皂苷Rg_(1)、Rb_(1)、Rk_(3)、Rh_(4),20(R)-人参皂苷Rg_(3)总含量为评价指标,Box-Behnken响应面法优化炮制工艺。建立乙酰苯肼(APH)-环磷酰胺(CTX)联合诱导的小鼠贫血模型,比较红三七、熟三七补血活性差异。结果最佳条件为蒸制温度130℃,蒸制时间4 h,烘干温度60℃,烘干时间48 h,总皂苷含量为8.326%,RSD为0.087%。药理活性表明,不同炮制工艺对三七补血活性有影响,红三七的补血活性优于熟三七。结论该方法稳定可行,可用于红三七的规范生产。
AIM To optimize the processing technology of red Notoginseng Radix et Rhizoma and evaluate its blood tonifying activity.METHODS On the basis of a single factor experiment,with steaming temperature,steaming time,drying temperature,and drying time as influencing factors,the total contents of notoginsenoside R 1,ginsenoside Rg_(1),Rb_(1),Rk_(3),Rh_(4),and 20(R)-ginsenoside Rg_(3)as evaluation indicators,Box-Behnken response surface method ology was used to optimize the processing technology.Upon the anemic mouse models jointly induced by 1-acetyl-2-phenylhydrazine(APH)and cyclophosphamide(CTX),the investigation of the blood tonifying activity of red Notoginseng Radix et Rhizoma was carried out in contrast to that of the steamed Notoginseng Radix et Rhizoma.RESULTS The optimal conditions,contributing saponin content of 8.326%and RSD of 0.087%,were determined as follows:steaming temperature of 130℃,steaming time of 4 hours,drying temperature of 60℃,and drying time of 48 h.The pharmacological activity revealed that the different processing techniques were responsible for the different blood enriching activity of notoginseng,with red Notoginseng Radix et Rhizoma displaying a better efficacy than that of steamed Notoginseng Radix et Rhizoma.CONCLUSION This stable and feasible method can be used to control the production of red Notoginseng Radix et Rhizoma.
作者
段嫏环
李起慧
吕冬
汪勇
崔秀明
DUAN Lang-huan;LI Qi-hui;L Dong;WANG Yong;CUI Xiu-ming(Faculty of Life Sciences and Technology,Kunming University of Science and Technology,Kunming 650500,China;Key Laboratory for Sustainable Utilization of Panax notoginseng Resources of Yunnan Province,Kunming 650500,China;Yunnan Research Institute for Panax notoginseng,Kunming 650500,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第1期48-54,共7页
Chinese Traditional Patent Medicine
基金
国家级人参属种质资源圃项目(2060302)
云南省重大科技项目(202202AG050021)。
关键词
红三七
炮制工艺
Box-Behnken响应面法
产地趁鲜加工
补血活性
red Notoginseng Radix et Rhizoma
processing technology
Box-Behnken response surface method
fresh processing in the place of origin
blood tonifying activity
