摘要
目的:观察兔膝骨关节炎(KOA)的组织病理变化并探讨其发病机制。方法:健康雄性新西兰兔膝关节强迫伸直位制动建立KOA模型,实验设对照组和模型组。4周后造模结束,取膝关节进行组织HE染色和免疫组织化学检测,观察并分析膝骨关节损伤及相关蛋白因子白细胞介素⁃1β(IL⁃1β)、基质金属蛋白酶⁃13(MMP⁃13)、带有血小板凝血酶敏感蛋白样模体的解整链蛋白金属蛋白酶⁃4(ADAMTS⁃4)的表达情况。结果:HE染色显示,模型组膝骨关节滑膜组织均存在不同程度增生、纤维化、淤血和炎性细胞浸润;软骨增生、有裂隙,软骨细胞排列紊乱、簇集,细胞形态多样,表层细胞纤维化等。IL⁃1β、MMP⁃13、ADAMTS⁃4在对照组、模型组软骨细胞均有表达,而模型组表达明显高于对照组。结论:关节软骨的退变、滑膜组织的炎症是KOA的病理学特征,其发病机制与软骨细胞IL⁃1β、MMP⁃13、ADAMTS⁃4蛋白表达异常有关。
Objective:To observe the histopathological changes and explore the pathogenesis of knee osteoarthritis(KOA)in rabbits.Methods:A KOA model was established in healthy New Zealand rabbits with knee joint forced extension and immobilization.The experiment was divided into the control group and model group.After 4 weeks of modeling,the knee joint was taken for HE staining and immunohistochemistry,which were used to observe and analyze knee joint injury and expression of related protein factors like interleukin⁃1β(IL⁃1β),matrix metalloproteinase⁃13(MMP⁃13)and a disintegrin and metalloproteinase with thrombospondin motifs⁃4(ADAMTS⁃4).Results:HE staining showed that there were various degrees of proliferation,fibrosis,congestion,and inflammatory cell infiltration in the synovial tissue of the knee joints in the model group,Cartilage hyperplasia,fissures,clusters of chondrocytes,diverse cell morphology,disordered arrangement,and fibrosis of surface cells.IL⁃1β、MMP⁃13 and ADAMTS⁃4 were expressed in both the control group and the model group of chondrocytes,but the model group of expression was significantly higher than that in the control group.Conclusion:The degeneration of articular cartilage and inflammation of synovial tissue are the pathological features of KOA,and the pathogenesis is related to the abnormal expression of IL⁃1β,MMP⁃13 and ADAMTS⁃4 in chondrocytes.
作者
罗娆珊
余立志
唐青梅
罗秉庆
梁伟明
LUO Raoshan;YU Lizhi;TANG Qingmei;LUO Bingqing;LIANG Weiming(The First Affiliated Hospital of Guangxi University of Science,Liuzhou 545000,China)
出处
《华夏医学》
CAS
2023年第5期24-28,共5页
Acta Medicinae Sinica
基金
广西科技基地和人才专项资助项目(桂科AD19245017)
广西科技大学博士基金项目(校科博20Z13)
广西卫生健康委员会自筹经费科研项目(Z20211376)。
关键词
膝骨关节炎
软骨组织损伤
发病机制
knee osteoarthritis
cartilagie tissue injury
pathogenesis
