摘要
为比较白芍不同极性部位的抗氧化活性,考察了其与总萜质量分数之间的相关性。将白芍提取物分为石油醚、乙酸乙酯、正丁醇、95%乙醇、65%乙醇和水6个不同极性部位,采用总还原力和DPPH、ABTS自由基清除能力3个指标评估其抗氧化性,并用显色法测定各部位的总萜质量分数。最后用SPSS 19.0软件分析白芍提取物抗氧化活性与总萜质量分数之间的相关性。实验结果表明,白芍95%乙醇提取部位的抗氧化性最强,其次是65%乙醇提取部位、正丁醇提取部位、乙酸乙酯提取部位、水提取部位、石油醚提取部位;此外,各部位总萜质量分数与抗氧化性强弱之间呈正相关,且与清除ABTS、DPPH自由基相关性达到显著。
The antioxidant activity of different polar parts of Paeonia Lactiflora are compared,and the correlation between antioxidant activity and total terpene content is explored.Paeonia Lactiflora extracts are divided into six polar parts such as petroleum ether,ethyl acetate,n-butanol,95%ethanol,65%ethanol and water,and their antioxidant activity is evaluated through total reduction power,and scavenging capacity for DPPH and ABTS free radicals.Total terpene content of various parts is determined by means of color reaction.SPSS 19.0 software is employed to analyze the correlation between antioxidant activity and total terpene content.Experiment results show that the antioxidant activity of 95%ethanol extract part of Paeonia Lactiflora is the strongest,comes next with 65%ethanol extract,n-butanol extract,ethyl acetate extract,water extract and petroleum ether extract.In addition,the content of total terpene is positively correlated with antioxidant activity,and is significantly correlated with the ability to scavenge ABTS and DPPH free radicals.
作者
杨颖
彭效明
居瑞军
杨思敏
尤聪聪
管洁
YANG Ying;PENG Xiao-ming;JU Rui-jun;YANG Si-min;YOU Cong-cong;GUAN Jie(Beijing Institute of Petrochemical Technology,Beijing 102617,China)
出处
《现代化工》
CAS
CSCD
北大核心
2024年第2期109-112,共4页
Modern Chemical Industry
基金
国家自然科学基金项目(21406015)
北京市教委科技计划一般项目(KM202210017011,KM202310017009)
北京石油化工学院校内学科平台建设项目(2019XK006)
北京石油化工学院交叉科研探索项目(BIPTCSF-019&021)
北京石油化工学院致远科研基金(2023013)
北京市科协青年人才托举工程项目(BYESS2023259)
大学生创新创业训练计划项目(2024J00293)。
关键词
白芍
提取物
抗氧化
极性部位
总萜质量分数
Paeonia Lactiflora
extracts
antioxidant
polar parts
total terpene content
